Table 1 Properties of Vph1-fusion proteins

Fluorescent	Excitation	Emission	Relative^{^a}	μ_bg^{^b}	LD_vph1^C	LD_cdc12	LD_whi5	GR_vph1^{^d}	GR_cdc12	GR_whi5	LD_Bleach50^{^e}
protein	filter	filter	brightness	at SNR = 4				at SNR = 4
tSapphire	390/18	525/50	41	54	1.3	6.4	50	100	99	40	3010
mAmetrine	438/24	525/50	35	43	1.5	7.0	54	100	99	57	6320
mTFP1	438/24	480/17	8.7	10	5.9	28	210	99	83	0.79	866
mTurquoise2	438/24	480/17	24	10	2.0	7.2	37	100	99	73	21200
sfGFP	488/6	525/50	100	20	0.4	1.6	6.5	100	100	100	5940
eGFP	488/6	525/50	100	20	0.52	1.5	6.5	100	100	100	15200
mNeongreen	488/6	525/50	210	20	0.26	0.7	2.6	100	100	100	6520
mNeongreen	504/12	542/22	120	8.8	0.37	1.2	4.2	100	100	100	3730
CitrineA206K	504/12	542/22	130	8.8	0.33	1.1	4.0	100	100	100	633
mRuby2	561/4	600/32	19	3.8	2.2	7.8	33	100	100	100	5320
tdKOkappa	546/10	577/25	120	3.1	0.39	1.1	3.8	100	100	100	4200
mKOk	546/10	577/25	57	3.1	0.80	2.4	8.7	100	100	100	7430
mKate2	600/14	655/40	14	4.8	3.0	11	55	100	100	100	2930
mCardinal	600/14	655/40	7.6	4.8	5.8	25	150	100	100	100	7320
mNeptune2	600/14	655/40	4.9	4.8	9.4	45	320	100	100	>89	9100