Table 1 Sequences and locations for primers used in genetic mapping of LPP1 | Marker name | Position | Forward primer | Reverse primer | Restriction enzymes to distinguish A17 and A20 alleles | Restriction enzymes to distinguish A17 and F83005 alleles |
|---|
| M1 | 12086486...12087011 | TCATCAATTCCGTCCAACAA | TATCCCTCGCGTATCCTCAC | Hpy188I | No polymorphism |
| M2 | 12109541...12109966 | AAATGGGAATGGATGGTTGA | ACTTGCATTGAAGGGGTGAC | AflIII | HinCII |
| M3 | 12816505...12816917 | GTAGTCGCCCATCATTGGTC | GAAACCGTCCTCGTTGATACA | TaqI | Dominant |
| M4 | 12971231...12971706 | CCTGTGCGATTGAAGTTGTTT | GCAATATTCCCTGAGCCAAG | HinfI | Hpy188I |
| M5 | 13100151...13100643 | CAATGGCACAATGGGTAAAA | CGGTGAGGAAAGAAAGTTAGAGA | AvaII | HincII |
| M6 | 13170690...13171159 | CCTGGCGAGTTTTGTTTTGT | AAAATGCAAATGCAACGACA | Tsp509I | BtgI |
| M7 | 13291032...13291523 | GAGCTTCCAAATACCCTTTCAA | TGCTAGCTTCCATGTTGTGC | BglII | AflII |
| M8 | 13294389...13294915 | CTGGCGTAGAGGATCACTGG | TGGGGGTGGTGAGAACTCTA | ApoI | ApoI |
| M9 | 13298918...13299373 | GTGATGTTCCACTTCAACACG | CATATCATAATAATGGGGGTTGG | PshAI | PshAI |
| M10 | 13309086...13309536 | AAAGTGGCCACCACAGTAGG | GAGAGGGAGAGGGGAGAATG | AseI | StyI |
| M11 | 13487194...13487665 | AATGACCACTGGGGGTTAGA | CGGTGATAATGATTTCCTTGC | BclI | No polymorphism |
| M12 | 13581669...13582122 | CCGAAGCTTGTGGACAATTA | CCACCGGTCACTGTCCTATT | BstYI | BstYI |
| M13 | 13606833...13607293 | TTGGTTGGAAATAAGTGTCAAGG | CCAAACAAGGGTAAACCAACA | AflIII/ | DraI |
| M14 | 13714308...13714799 | CATCGACCTTGATAGCCACA | AGTGCAGACGCTTACCACAA | HpyCH4V | HpyCH4V |
| M15 | 13807044...13807513 | GAAAGCCACCGGTCAGAGTA | CCTTGGACGAATAATTGAGACA | Size polymorphism | Size polymorphism |
| M17 | 13854196...13854645 | AATACCAAATGGAGGGTGGA | ATAAAGGCACAATTGGCAAAC | Size polymorphism | Size polymorphism |
| M18 | 13856315...13856782 | TGTTCACTTCCAAAGGATTTCA | GGCATGACTATGTGGGACCT | Size polymorphism | Size polymorphism |
| M20 | 14304994...14305467 | AACCTCTAAACGGCCAAGGT | GTGCAGGAGCATCAGACAAA | Size polymorphism | Size polymorphism |
| M21 | 14552501...14552956 | CGCAGAGGATACCCGTTTAC | CAAGTTGGGAAAATAGGGTGTT | No polymorphism | BsaBI |