Table 3 Full-length sequence statistics
SamplescDNA SizeReads of InsertNumber of five prime readsNumber of three prime readsNumber of poly-A readsNumber of filtered short readsNumber of non-full-length readsNumber of full-length readsNumber of full-length non-chimeric readsAverage full-length non-chimeric read lengthFull-Length Percentage (FL%)Artificial Concatemers (%)
F011-2K197,712122,794133,392129,50324,31867,847105,547104,7641,23153.38%0.74%
F012-3K185,044121,426127,171125,65710,99869,849104,197103,9672,29056.31%0.22%
F013-6K127,38173,95177,97276,2088,72959,60759,04558,9352,90646.35%0.19%
F01All510,137318,171338,535331,36844,045197,303268,789267,6662,01152.69%0.42%
F021-2K230,897130,863147,167142,22236,77785,056109,064108,4661,24247.23%0.55%
F022-3K180,575104,783111,538110,08014,47779,28686,81286,6602,30048.08%0.18%
F023-6K141,35791,81595,44294,6544,46856,96279,92779,7893,29956.54%0.17%
F02All552,829327,461354,147346,95655,722221,304275,803274,9152,17249.89%0.32%
  • cDNA size: insert fragment size of cDNA libraries; Reads of insert: the number of ROI sequences; Number of 5′ reads: the number of ROI sequences containing 5′ ends; Number of 3′ reads: the number of ROI sequences containing 3′ ends; Number of poly-A reads: the number of ROI sequences containing poly-A sequence; Number of filtered short reads: the number of filtered ROIs of <300 bp; Number of non-full-length reads: the number of non-full-length ROIs; Number of FLNC reads: the number of full-length non-chimeric ROIs;Average FLNC read length: average length of full-length non-chimeric sequences; Full-length percentage (FL%): the percentage of ROIs that were full-length sequences;Artificial concatemers (%): the percentage of ROIs that were full-length chimeric sequences.