Table 1 Summary of embryonic and larval lethal interactions between ngn-1(ok2200), cnd-1 and canonical axon guidance and cell migration mutants. *P < 0.005; **P < 0.001; ***P < 0.0001, 2-tailed student t-test and Bonferroni correction for multiple comparisons. Note that double mutants were compared against the single mutant that showed the strongest phenotype. ngn-1; vab-1 and ngn-1; sax-3 double mutants could not be isolated away from balancer chromosomes or rescuing transgenes suggesting 100% embryonic lethality in the unbalanced strains
GenotypeEmbryonic Lethality (SD)Larval Lethality (SD)BroodsN
wild-type0.11 (0.2)0.12 (0.2)4823
ngn-1(ok2200)45.9 (4.8) ***10.2 (2.2) *3482
ngn-1; ngn-1::GFP9.8 (10.6) ***ND5471
cnd-1(ju29)1.5 (1.4)1.0 (1.2)4783
ngn-1; cnd-145.5 (6.1)26.1 (5.2)81967
cnd-1(gk718)7.9 (1.4)12.4 (2.0)61464
ngn-1; cnd-1(gk718)51.7 (5.5)18.7 (1.1) **6851
daf-18(ok480)3.3 (1.0)0.3 (0.5)61586
ngn-1 daf-18(ok480)64.3 (5.4)17.1 (5.6)4715
sdn-1(zh20)7.7 (5.3)5.5 (3.7)5868
ngn-1; sdn-1(zh20)11.8 (8.5) *10.4 (5.9)5656
efn-4(bx80)14.3 (2.5)8.0 (3.3)6927
ngn-1 efn-4(bx80)60.1(15.3)13.1(1.9)3389
mab-20(bx24)15.2 (7.2)5.0 (4.1)5905
ngn-1 mab-20(bx24)32.3 (7.3)3.1 (1.0) *51510
vab-1(dx31)6.7 (5.0)18.4 (4.1)5763
ngn-1; vab-1(dx31)NDND
sax-3 (ky123)70.1 (17.2)10.3 (8.2)3635
ngn-1; sax-3(ky123)NDND