Table 1 Injection data and comparison of ECFP mutation by adult and embryo injections
Injection MixG-1G0 Fluorescence PhenotypeG1 Fluorescence Phenotype
ProteinsgECFPSaponinG-1 CrossNo. InjectedInjection time PBM (hours)No. on EC dayNo.Cyan+Cyan-Cyan-Cyan MosaicG0 C+R+ OutcrossNo.Cyan+Cyan-Cyan-
DsRed+DsRed-DsRed+DsRed+DsRed+DsRed-DsRed+
Adult injections: P2C-Cas9/gRNA mix injections into VgCp26.10 colony females (G-1) crossed with wild type males
0.5 µg/µL0.75 µg/µL+VgCp26.10 Females X WT Males300242124251063172 (0.47%)0Female2951031920
Male6223242980
0.75 µg/µL1.2 µg/µL+VgCp26.10 Females X WT Males8348601941602410 (5.2%)1* (1.2%)Female5226260
Male102385311 (10.8%)
0.75 µg/µL1.2 µg/µL+VgCp26.10 Females X WT Males634733161521072 (1.2%)1** (0.62%)Female6129320
Male8435490
0.75 µg/µL1.2 µg/µL+VgCp26.10 Females X WT Males78464671413002** (2.8%)Female11330830
MaleND
Sap+ Totals:52435185135947814 (1.7%)4 (0.47%)132958573311 (0.82%)
0.75 µg/µL1.1 µg/µLVgCp26.10 Females X WT Males9728781841147000Female217961183 (1.4%)
Male9749480
0.75 µg/µL1.1 µg/µLVgCp26.10 Females X WT Males7125431736111200Female4115260
Male207109980
Sap - Totals:168121357175182005622692903 (0.53%)
Totals:6924721208534660001891854102314 (0.74%)
Embryo injections: Cas9/gRNA mix injections into homozygous VgCp26.10 transgenic embryos (G0)
0.2 µg/µL0.075 µg/µLVgCp26.10 Intercross309Not Screened232023173 (0.13%)0
0.2 µg/µL0.3 µg/µLVgCp26.10 Intercross382Not Screened5646550652 (0.92%)88 (1.5%)
Totals:6917966782355 (0.69%)88 (1.1%)
  • * individual had one cyan+ eye and one cyan- eye.

  • ** these individuals had markedly reduced cyan fluorescence.