Table 2 Confocal microscope settings for imaging transgenic D. melanogaster with S3a-series+yBE0.6 enhancer fluorescent protein reporter transgenes
Fluorescent ProteinEGFPmCerulean-NLSmCherry-NLSE2-Crimson-NLS
Laser (nm)488458543633
Laser%10202015
HV700850850750
Gain1211
Offset120105
Aperture200200200200
Step Size (μm)10101010
Excitation Filters485-595 (512)450-585 (476)550-648 (580)620-780 (665)
Emission Filters500-530462-485555-625Far Red