Table 3 Confocal microscope settings for imaging transgenic D. melanogaster with Red Light/Green Light-series dual reporter transgenes
Fluorescent ProteinEGFPDsRed.T4-NLSE2-Crimson-NLS
Laser (nm)488543633
Laser%101515
HV600750700
Gain111
Offset115
Aperture200200200
Step Size (μm)101010
Excitation Filters485-595 (512)550-648 (580)620-780 (665)
Emission Filters495-530575-640Far Red