Table 1 Primers used to clone D. melanogaster enhancers for cloning into reporter transgene vectors
CRE∼SizePrimerSequence
yBE0.6600BE2.5 FwdTTCCGggcgcgccCTGTGGGTGCAATGATTTAGAATG
BE3.5 RvsTTGCCcctgcaggGTTATTGGCAGGTGATTTTGAGC
t_MSE2350tan_MSE-mid-FTTCCGggcgcgccTGAAATAATAATAAATAATCAGAAT
tan_MSE-mid-RTTGCCcctgcaggTGTTTCAACTCAATCCTAGCAGTTGG
dimorphic690DE core FwdTTCCGggcgcgccTCGCCTccgcggCTCTTTCTCTTTGCCATTTTAAC
element coreDE core RvsTTGCCcctgcaggCCCTTGgctagcGTGTGTGAACCAATTTGTTGTGC
LAE1,400bab TRE FwdTTCCGggcgcgccGTGAGGGGCAAATTATGGAGAG
bab TRE RvsTTGCCcctgcaggGTGCGCCTAACTAGCCAACAATTAG
Expanded Dimorphic1,580sub1orthoF1TTCCGggcgcgccCACATAAAAATCAGCAACAAASTTGC
Elementdimorphic Rvs1TTGCCcctgcaggCAAAACKGCRCATAAAAMSAAATTACA
  • Notes:

  • 1. ‘ggcgcgcc’ and ‘cctgcagg’ are sequences recognized respectively by the AscI and SbfI restriction endonucleases. These restriction enzyme sites were used to clone PCR amplified sequences into the pRLGL8 reporter vector. ‘ccgcgg’ and ‘gctagc’ are sequences recognized respectively by the SacII and NheI restriction endonucleases.

  • 2. The approximate PCR product sizes are reported in base pairs (bp).

  • 3. ‘S’, ‘K’, ‘R’, and ‘M’ are IUPAC notations for degenerate bases. S = G or C, K = G or T, R = A or G, and M = A or C.