Table 2 Donor cassette plasmids used in this study
NameDescription
Cassette 1 for 4-cassette or 5 cassette system (5′-TGG ...... 3′-TAC)
pXF121mex-5 promoter
pSDH60spe-11 promoter
Cassette 2 for 4-cassette or 5-cassette system (5′-ATG-3′ ...... 3′-CCA-5′)
Tags
pXF89halotag (no STOP codon, PATC introns)
pJF5gfp (no STOP codon, PATC introns)
pXF222mkate2 (no STOP codon)
pSDH61epdz (no STOP codon)
pSM10mscarlet (no STOP codon)
pSM12dendra2 (no STOP codon)
Genes
pJF7tomm-20 (no STOP codon)
pSDH50tomm-20 (aa 1-55) (no STOP codon)
pXF262cox-4 (no STOP codon)
pXF250npp-20 (no STOP codon)
Cassette 3 for 4-cassette system (5′-GGT-3′ ...... 3′-ATT-5′)
Tags
pXF88halotag (includes STOP codon, PATC introns)
pJF6gfp (includes STOP codon, PATC introns)
pXF130mkate2 (includes STOP codon)
pSM08mscarlet (includes STOP codon)
pSM03dendra2 (includes STOP codon)
ORFs
pXF90halotag::hdel (includes STOP codon, PATC introns)
Cassette 3A for 5-cassette system (5′-GGT-3′ ...... 3′-TGC-5′)
Tags
pSDH51halotag (no STOP codon, PATC introns)
pSM04mkate2 (no STOP codon)
pSDH57mscarlet (no STOP codon)
Cassette 3B for 5-cassette system (5′-ACG-3′ ...... 3′-ATT-5′)
Tags
pXF276lov domain (includes STOP codon)
pSDH52epdz (includes STOP codon)
pSM05mkate2 (includes STOP codon)
Cassette 4 for 4-cassette or 5-cassette system (5′-TAA-3′ ...... 3′-CAT-5′)
pXF85pie-1 3′UTR
pSDH54tbb-2 3′UTR
pSDH66unc-54 3′UTR