Table 4 MosSCI targeting vectors used in this study
NameCommentsAssembly
pXF87MosSCI backboneDerived from pCFJ350
Donor vectors used for assemblyAssembly efficiency
CassettesDigestionSequencing
1234
pJF13ER lumen, HalotagpXF121XF17F/RapXF90pXF854/52/2
pJF17Mitochondrial OM, HalotagpXF121pJF7pXF88pXF854/51/2
pXF253ERES + nuclear pores (NPP-20), GFPpXF121pXF 250pJF6pXF854/62/2
pXF255ERES + nuclear pores (NPP-20), HalotagpXF121pXF 250pXF88pXF855/62/2
pXF266Mitochondrial matrix, HalotagpXF121pXF 262pXF88pXF851/41/1
pSM20Mitochondrial OM, mKate2pXF121pJF7pXF130pXF854/52/2
pSM22Mitochondrial OM, mScarletpXF121pJF7pSM08pXF854/52/2
pSM17Mitochondrial OM, Dendra2pXF121pJF7pSM03pXF854/52/2
pSM16Mitochondrial OM, GFPpXF121pJF7pJF6pXF852/52/2
123A3B4
pSDH68Mitochondrial OM, Halotag, LOVpXF121pSDH50pSDH51PCR fragmentpSDH5411/152/2
  • a Annealed oligos.