Table 1 Summary of the events leading to URA3 loss
Strains*Genetic events
pop-out (URA3 deletion)Interhomolog recombination (GC/XO/BIR)Chromosome lossChromosome TruncationEctopic TranslocationMutational Inactivation
CAGL1A (wt, A; 1)100% (24)
CAGL1A (wt, A; 2)90% (18)10% (2)
CAGL1B (wt, B; 1)92% (22)8% (2)
CAGL1B (wt, B; 2)90% (19)10% (1)
CAGL2A (rad59, A; 1)85% (17)10% (3)
CAGL2A (rad59, A; 2)95% (19)5% (1)
CAGL2B (rad59, B; 1)83% (20)12% (3)5% (1)
CAGL2B (rad59, B; 2)90% (17)10% (2)5% (1)
CAGL3A (rad51, A; 1)65% (13)10% (3)20% (4)
CAGL3A (rad51, A; 2)73% (22)3% (1)17% (5)7% (2)
CAGL3B (rad51, B; 1)85% (17)5% (1)15% (2)
CAGL3B (rad51, B; 2)67% (20)3% (1)27% (8)3% (1)
CAGL4A (rad52, A)90% (9)10% (1)
CAGL4.1A (rad52, A)12,5% (1)87,5% (7)
CAGL4B (rad52, B)20% (4)30% (6)50% (10)
CAGL5A (rad59 rad52, A; 1)10% (1)90% (9)
CAGL5A (rad59 rad52, A; 2)35% (7)65% (13)
CAGL5B (rad59 rad52, B; 1)11% (1)67% (6)22% (2)
CAGL5B (rad59 rad52, B; 3)5% (2)15% (3)75% (15)
CAGL6B (lig4 rad52, B; 1)4% (1)46% (11)50% (12)
CAGL6B (lig4 rad52, B; 2)10% (3)30% (6)55% (11)5% (1)
CAGL01 (lig4, A; 1)100% (20)
CAGL4A-FRT25% (5)75% (15)
CAGL4B-FRT10% (2)55% (11)45% (9)
CAGL5B**-FRT15% (3)20% (4)65% (13)
CAGL5B-FRT25% (5)40% (8)35% (7)
CAGL6B-FRT5% (1)55% (11)40% (8)
CAGL2776% (38)22% (11)2% (1)
CAGL2881% (30)19% (7)
  • For each strain (except CAGL27 and CAGL28) both genotype and test allele (A or B) are indicated. 1 and 2 refers to independent experiments. The number of derivatives analyzed in each experiment is shown in parenthesis. Genetic events are indicated at the top. For strains CAGL1, CAGL2 and CAGL3, 5FOAR derivatives resulting from URA3 pop-out conserved the wild type RAD52 allele and the disrupted rad52::hisG-URA3-hisG allele had been processed to rad52::hisG. Derivatives resulting from IHR carried only the wild type RAD52 allele (2 copies). CL and CT were further confirmed by SNP RFLP analysis and CHEF Southerns. nd-not determined. 5FOAR derivatives from FRT strains were screened for SSA, SNPs GRCs. Strain CAGL5B**-FRT was intended to be CAGL5A-FRT (tester A) since it was derived from CAGL2A but behaved as if carrying RAD52B as the test allele. It is likely that a reciprocal exchange between both RAD52 alleles (gene conversion or crossover) occurred at some step during its generation.