Table 1 List of transformants generated
Transformed constructBackground strainDetails of the native chromosome that the construct derives fromConstruct Details
C-terminus 6x histidine-tagged ctg20822.0.g90 (AL1) aAL1 chromosome deletion line #5 bCtg20822.0 contains 1 gene: Alba-like 1C-terminus 6x histidine tag with linker (GSGGSG) after AL1 protein
C-terminus 10x histidine-tagged ctg20822.0.g90 (AL1)AL1 chromosome deletion line #4 bCtg20822.0 contains 1 gene: Alba-like 1C-terminus 10x histidine tag with no linker after AL1 protein
N-terminus 6x histidine-tagged ctg20822.0.g90 (AL1)AL1 chromosome deletion line #5 bCtg20822.0 contains 1 gene: Alba-like 1N-terminus 6x histidine tag with linker (GSGGSG) prior to AL1 protein
C-terminus MYC-tagged ctg15169.0.g18 (AL2)JRB310Ctg15169.0 contains 2 genes: Alba-like 2 gene, and a predicted gene containing an EF hand motifC-terminus MYC tag with linker (8x glycine) after AL2 protein
GFP in ctg18685.0 nanochromosome (Hsp70) aJRB310Ctg18685.0 contains 1 gene: Hsp70Ciliate codon-corrected GFP replacement of the Hsp70 gene on the Hsp70 nanochromosome
Mango RNA aptamer in ctg4739.0JRB310Ctg4739.0 contains 1 gene containing a predicted Alba domainMango RNA replacement of the ctg4739.0 gene on the ctg4739.0 nanochromosome
  • a Transformants further characterized in this paper.

  • b See Clay et al. 2019.