Table 1 Quality filtering and statistics of raw reads obtained in transcriptome libraries of B. napus exposed to low-temperature treatments
Sample nameRaw readsClean readsclean basesError rate (%)Q20 (%)Q30 (%)GC content (%)
HX17_MA161608094589665728.84G0.0397.3692.9344.06
HX17_MA246842038452522726.79G0.0396.9892.1744.39
HX17_MA351620482504059007.56G0.0397.1192.0145.91
HX17_CA145028428440965126.61G0.0396.9791.6645.83
HX17_CA253540226526331747.89G0.0396.7291.4946.35
HX17_CA349776538479623907.19G0.0396.7091.5443.39
HX17_FA146114476453909926.81G0.0396.6691.3744.84
HX17_FA266471914644427669.67G0.0397.1192.4045.85
HX17_FA349756338491913307.38G0.0397.2092.5345.64
HX17_MB145873106450289406.75G0.0395.4288.1744.09
HX17_MB247889494470671407.06G0.0396.7591.5745.00
HX17_MB346228026453443946.80G0.0396.9492.0444.25
HX17_CB1701497746846786010.27G0.0397.8594.1244.40
HX17_CB254975576539776128.10G0.0397.1292.0444.50
HX17_CB355136028538407128.08G0.0397.0291.8244.74
HX17_FB1795186807816330611.72G0.0397.7093.7044.76
HX17_FB248126142471475087.07G0.0396.7591.2844.80
HX17_FB352485614515277627.73G0.0397.7793.7944.19
HX58_MA167391320657270889.86G0.0397.1692.1644.28
HX58_MA266677164647761529.72G0.0397.0991.9843.86
HX58_MA347017928448923306.73G0.0397.0792.0743.88
HX58_CA144491830431284446.47G0.0396.6691.3946.46
HX58_CA247877742462437186.94G0.0396.7391.4045.62
HX58_CA349056666475941847.14G0.0397.8593.9945.77
HX58_FA1778254807608064611.41G0.0397.9294.0844.84
HX58_FA2727148827067529610.6G0.0397.7293.6145.33
HX58_FA362524530607875349.12G0.0397.7193.5945.10
HX58_MB155247676540981928.11G0.0396.7491.2344.28
HX58_MB257837196567646488.51G0.0396.8291.3445.16
HX58_MB349353542482258987.23G0.0397.3492.5444.69
HX58_CB161754716600618409.01G0.0397.8893.9543.42
HX58_CB266709668646723489.70G0.0397.6993.6042.76
HX58_CB352648416514005087.71G0.0396.7391.2843.77
HX58_FB158213716569780608.55G0.0397.0491.8946.07
HX58_FB259190884577299828.66G0.0397.1192.3746.39
HX58_FB360769766591894688.88G0.0397.1092.3645.06
Assembly StepsTotal assembled transcriptsNumber of transcripts (>1000bp)Percent of transcriptsMaximum transcript lengthAverage transcript lengthN50 value (bp)Coverage (X)
Assembler SOAPdenovotrans (65 k-mer)91,76532,50935.4312,805903.391,427160
After gap filling and hierarchical clustering53,80624,42945.4012,6741,096.961,499218
After removing mis-assembled transcripts46,55619,34741.559,8111,013.661,393226
BLASTX best group representatives (unigenes)25,40012,58849.569,8111,153.301,502240