Table 4 NAT haplotype frequencies (%) estimated in the different species and sub-species of the genus Pan and results of Hardy-Weinberg equilibrium tests.
Pan species and sub-species
P. t. verus (Western chimpanzee)
San Diego sampleaBPRC sampleP. t. ellioti (Nigeria-Cameroon chimpanzee)P. t. troglodytes (Central chimpanzee)P. t. schweinfurthii (Eastern chimpanzee)P. paniscus (Bonobo)
Pan NAT1 haplotypes
NAT1*179.33 (1.71)80.4365.0070.0066.7025.00
NAT1*27.97 (0.94)4.355.00000
NAT1*31.59 (1.38)0010.008.330
NAT1*48.33 (0)13.040000
NAT1*52.78 (0)00000
NAT1*602.1700053.60
NAT1*70010.00000
NAT1*800010.0000
NAT1*900008.330
NAT1*100020.0010.008.330
NAT1*1100008.330
NAT1*120000021.40
Total (2n chromosomes)364620101228
Hardy-Weinberg testb:
Ho0.36 (0.03)0.260.500.600.500.71
He0.37 (0.03)0.340.550.530.580.63
P-value∈ [0.39 ; 0.64]0.200.35> 0.990.510.45
Pan NAT2 haplotypes
NAT2*192.49 (1.27)91.305.0010.0000
NAT2*22.41 (0.94)4.350000
NAT2*302.170000
NAT2*45.10 (1.03)010.0080.0091.700
NAT2*502.170000
NAT2*60085.0010.0000
NAT2*70000089.3
NAT2*8000003.57
NAT2*9000007.14
NAT2*1000008.330
Total (2n chromosomes)364620101228
Hardy-Weinberg testb:
Ho0.15 (0.03)0.170.300.200.170.21
He0.15 (0.02)0.170.280.380.170.20
P-value∈ [0.08 ; > 0.99 ]> 0.99> 0.990.11> 0.99> 0.99
Pan NATP haplotypes
NATP*124.61 (2.46)44.00010.0000
NATP*252.14 (1.7)42.0040.0010.0058.300
NATP*300020.0000
NATP*406.000000
NATP*502.000000
NATP*62.32 (1.03)00000
NATP*719.33 (2.21)6.000000
NATP*81.59 (1.38)025.0020.008.330
NATP*900030.0000
NATP*1000008.330
NATP*110010.00016.670
NATP*12005.00000
NATP*13 c000000
NATP*14005.00000
NATP*150015.00000
NATP*1600010.0000
NATP*1700008.330
NATP*180000096.43
NATP*19000003.57
Total (2n chromosomes)365020101228
Hardy-Weinberg testb:
Ho0.64 (0.04)0.600.900.600.670.07
He0.65 (0.01)0.640.780.890.670.07
P-value∈ [0.09 ; > 0.99 ]0.060.03 d0.150.76> 0.99
  • a Average over the 122 sub-samples (see text), standard deviation in brackets.

  • b Test for departure from Hardy-Weinberg equilibrium; Ho: observed heterozygosity, He: expected heterozygosity (equivalent to gene diversity). The only significant deviation from equilibrium (heterozygote excess at NATP in P. t. ellioti) is shown in bold.

  • c Haplotype NATP*13, which combines SNPs at 170, 253, 289, 386, 499, 633, and 656 (Table 3), was inferred only for the genotype of the hybrid P. t. verus/troglodytes individual.

  • d P-value > 0.05 after correction for multiple testing.