Table 2 The editing types of all edited individuals in three batches of experiments
Injection siteIDTarget geneEditing type (number)Editing Rate
Ventral cephalic side1AMrjp1Wild-type(12) M15(8)8/20 = 40%
2AMrjp1Wild-type (14) M12(1) M15(5)6/20 = 30%
3AMrjp1M1(14) M2(1) M3(1) M6(3) M15(1)20/20 = 100%
4AMrjp1M8100%
5AMrjp1M6100%
6AMrjp1Wild-type(8)M8(12)12/20 = 60%
8AMrjp1M6100%
9AMrjp1M5(12) M8(8)20/20 = 100%
10AMrjp1M4(14) M13(6)20/20 = 100%
11AMrjp1M8100%
12AMrjp1M11(2) M15(18)20/20 = 100%
13AMrjp1M6100%
14AMrjp1M8(14) M9(5) M14(1)20/20 = 100%
15AMrjp1M8(10) M10(10)20/20 = 100%
1DPax6Wild-type(1) P6(19)19/20 = 95%
2DPax6P11100%
3DPax6Wild-type(1) P7(16) P14(3)19/20 = 95%
4DPax6P9(2) P16(18)20/20 = 100%
5DPax6P7(16) P15(4)20/20 = 100%
6DPax6P1(3) P7(17)20/20 = 100%
7DPax6P4(17) P5(1) P7(1) P10(1)20/20 = 100%
8DPax6P7(1) P3(19)20/20 = 100%
9DPax6P7100%
10DPax6P7(1) P8(1) P13(18)20/20 = 100%
11DPax6P2(10) P7(1) P13(9)20/20 = 100%
12DPax6P12(20)20/20 = 100%
13DPax6Wild-type(1) P7(19)19/20 = 95%
Dorsal posterior side6CMrjp1Wild-type(19) M7(1)1/20 = 5%
8CMrjp1Wild-type(14) M6(6)6/20 = 30%
  • Note: 4A, 5A, 8A, 11A, 13A, 2D, 9D and 12D were biallelic homozygous mutants; 3A, 9A, 10A, 12A, 14A, 15A, 4D, 5D, 6D, 7D, 8D, 10D and 11D were biallelic heterozygous mutants.