Table 2 Ahringer clones causing RNAi effects on GFP-labeled canal length in RNAi-sensitized animals
GeneNematode Gene AffectedMost Frequent Canal RNAi Phenotype# canals examined in later tests% mutant canalsAvg. mutant canal lengthStd. Dev. of mutantsp-value vs.wild-type
Strain BK540Control: wild-type canals1000%4.0±0.0
Knockdowns exhibiting effects with high statistical confidence:
ceh-6*K02B12.1large fluid-filled cysts33100%1.1±0.356.7x10−24
exc-10T25C8.1large fluid-filled cysts6368%3.3±0.392.7x10−24
egal-1C10G6.1medium-sized fluid-filled cysts40100%1.1±0.375.7x10−36
mop-25.2*Y53C12A.4medium-sized fluid-filled cysts, vesicles along swollen cytoplasm20100%1.1±0.223.4x10−23
exc-11F41E7.1medium-sized fluid-filled cysts3497%1.7±0.521.4x10−30
exc-12T05D4.3medium-sized fluid-filled cysts5379%2.6±0.599.4x10−28
best-3C01B12.3swollen luminal tip3628%3.4±0.315.8x10−7
gck-3*Y59A8B.23swollen tip, vesicles, convolutions7657%3.5±0.261.6x10−20
fbxa-183F44E7.6swollen tip with vesicles12566%3.1±0.273.0x10−30
exc-14K11D12.9vesicles along swollen cytoplasm36100%1.4±0.479.5x10−34
exc-15T08H10.1vesicles along swollen cytoplasm4127%3.5±0.154.3x10−7
exc-13C03G6.5vesicles along swollen cytoplasm7919%3.5±0.321.2x10−6
cyk-1*F11H8.4vesicles along swollen cytoplasm7777%3.1±0.282.8x10−34
inx-12*ZK770.3periodic cytoplasmic beads4696%2.7±0.502.2x10−35
inx-13*Y8G1A.2periodic cytoplasmic beads5080%2.8±0.513.3x10−29
ceh-37*C37E2.5periodic cytoplasmic beads4030%3.3±0.317.7x10−8
mxt-1Y18D10A.8cytoplasmic beads with vesicles5993%2.6±0.512.0x10−38
vha-5*F35H10.4beads, vesicles, swollen cytoplasm7859%2.5±0.797.7x10−22
Knockdowns exhibiting effects with lower statistical confidence:
dhhc-2Y47H9C.2cytoplasmic beads with vesicles5721%3.5±0.212.3x10−6
T19D12.9swollen tip, vesicles, convolutions8326%3.3±0.471.3x10−6
gst-28Y53F4B.31swollen tip with vesicles2627%3.4±0.387.8x10−6
gsr-1C46F11.2swollen tip, vesicles, convolutions735%3.4±0.300.030
H09G03.1vesicles along swollen cytoplasm1797%3.3±0.464.7x10−4
C09F12.3vesicles along swollen cytoplasm888%3.3±0.224.3x10−3
exc-5 (qp110)large cysts at canal tips118100%1.35±1.35
Knockdowns exhibiting suppression effects on exc-5:p-value vs. exc-5
suex-1F12A10.7suppresses exc-5 mutant cysts22495%2.25±0.651.3x10−18
suex-2C53B4.1suppresses exc-5 mutant cysts20797%2.26±0.731.7x10−14
  • Knockouts of genes found to have effects on C. elegans excretory canals. Thick lines separate knockdowns that showed effects with high confidence from knockdowns with effects of lower confidence, and knockdowns that exerted suppression effects on exc-5 mutants. Thin lines separate genes by the most common phenotype exhibited in knockdowns, and correspond to panels in Figures 2, 3, 4, 5, and 6, respectively. Asterisks indicate genes previously demonstrated to have effects on the excretory canals. p-value was determined via 3x2 Fisher’s exact test (see Materials & Methods), with 1x10−6 viewed as determining high significance (exc-13 was included as being close to this threshold, plus showing effects in “million mutation” animals).