Table 1 List of strains used in this study, with genotype descriptions
STRAINGENOTYPEDESCRIPTIONREFERENCE
BK36unc-119(ed3) III; qpIs11 [unc-119; Pvha-1::gfp] IN2 with integrated GFP marker expressed in excretory canal cytoplasm(Mattingly and Buechner 2011)
BK540rrf-3(pk1426) II; qpIs11 [unc-119; Pvha-1::gfp] IRNAi-sensitized strain expressing GFP in canalsThis study
BK541sid-1(pk3321) II; qpIs11 [unc-119; Pvha-1::gfp] ISystemic RNAi-impaired strain expressing GFP in canalsThis study
BK542exc-2(rh90) X; qpIs11 [unc-119; Pvha-1::gfp] Iexc-2(rh90) expressing GFP in canalsThis study
BK543exc-3(rh207) X; qpIs11 [unc-119; Pvha-1::gfp] Iexc-3(rh207) expressing GFP in canalsThis study
BK544exc-4(rh133) qpIs11 [unc-119; Pvha-1::gfp] Iexc-4(rh133) expressing GFP in canalsThis study
BK545exc-5(rh232) IV; qpIs11 [unc-119; Pvha-1::gfp] Iexc-5(rh232) expressing GFP in canalsThis study
BK546exc-7(rh252) II; qpIs11 [unc-119; Pvha-1::gfp] Iexc-7(rh252) expressing GFP in canalsThis study
BK547BK540; exc-2(rh90) Xexc-2(rh90) expressing GFP in canals in RNAi-sensitized backgroundThis study
BK548BK540; exc-3(rh207) Xexc-3(rh207) expressing GFP in canals in RNAi-sensitized backgroundThis study
BK549BK540; exc-4(rh133) Iexc-4(rh133) expressing GFP in canals in RNAi-sensitized backgroundThis study
BK550BK540; exc-5(rh232) IVexc-5(rh232) expressing GFP in canals in RNAi-sensitized backgroundThis study
VC20239exc-15(E89K) mutation“million mutation” strain homozygous at ∼3-6 loci(Thompson et al. 2013)
VC20363H09G03.1(P15S) mutation“million mutation” strain homozygous at ∼3-6 loci(Thompson et al. 2013)
VC20573H09G03.1(G67R) mutation“million mutation” strain homozygous at ∼3-6 loci(Thompson et al. 2013)
VC40373exc-13(C44Y) mutation“million mutation” strain homozygous at ∼3-6 loci(Thompson et al. 2013)
VC40556T19D12.9(Q61X) mutation“million mutation” strain homozygous at ∼3-6 loci(Thompson et al. 2013)
VC40788C09F12.3(P41S) mutation“million mutation” strain - Died at thaw; not used(Thompson et al. 2013)