Table 3 Chromosome copy numbers in T. thermophilus HB27
Gene locusReal-time PCRaFluorescent microscopyb
TTC1610 (oriC region)7.87 ± 0.737.53 ± 2.71
TTC0574 (relatively closer to terC)7.01 ± 1.55/
  • a real-time PCR method was used to measure the chromosome copy numbers.

  • b Fluorescent microscopy was used to count ParB-sGFP fluorescent foci numbers per cell reflecting the copy numbers of the chromosomal origin region (100 cells were counted).