Table 1 S. pombe strains used in this study
StrainGenotypeSource and reference
SP6h-leu1-32Laboratory stock
MS01h-leu1-32 transformed with pREP41-GFP-H3.3This work
MS02h-leu1-32 transformed with pREP41-GFP-cnp1This work
MS03h-leu1-32 cnp3-1 transformed with pREP41-H3.3This work
MS04h-leu1-32 cnp3-1 transformed with pREP41-cnp3-1This work
MS168h+leu1-32::nmtGFP-cnp1-leu1+ cnp3::kanR ade6-M216This work
MS213h-leu1-32 lys::nmtGFP-cnp1-lys1+ cnp3-mCherry-leu1+This work
MS216h+leu1-32::nmtGFP-cnp1-leu1+ ade6-M216This work
MS217h-leu1-32 lys::nmtGFP-cnp1-lys1+ cnp3-1-mCherry-leu1+This work
MS262h+leu1-32::nmtGFP-cnp1-leu1+ cnp3-1 ade6-M216This work
MS343h-leu1-32 GFP-cnp1-hphLaboratory stock
MS344h-leu1-32 GFP-cnp1-hph cnp3-1This work
MS413h-leu1-32 cnp3-1 ade6-M210 ch16This work
MS468h-leu1-32 cnp3-GFP-leu1+This work
MS497h-leu1-32 GFP-cnp1-hph mis16-53This work
MS503h-leu1-32 GFP-cnp1-hph mis18-818This work
MS508hleu1-32 GFP-cnp1-hph mis6-302This work
MS525h-leu1-32 lys::nmtGFP-cnp1-lys1+ cnp3-3HA 6his-leu1+This work
MS527h-leu1-32 lys::nmtGFP-cnp1-lys1+ cnp3-1-3HA 6his-leu1+This work
MS532h-leu1-32 GFP-cnp1-hph mis6-302 cnp3-1This work
MS533h-leu1-32 GFP-cnp1-hph mis16-53 cnp3-1This wor
MS534h-leu1-32 GFP-cnp1-hph cnp3-1 mis18-818This work
MS544h-leu1-32 cnp3-1-GFP-leu1+This work
MS545h-leu1-32 GFP-cnp1-hph cnp3-1mCherry-leu1+ mis18-818This work
MS546h-leu1-32 GFP-cnp1-hph cnp3-mCherry-leu1+ mis18-818This work
MS548h-leu1-32 GFP-cnp1-hph cnp3-mCherry-leu1+This work
MS549h-leu1-32 GFP-cnp1-hph cnp3-1-mCherry-leu1+This work
MS568h-leu1-32 Sad1-mCherry<<kanR transformed with pREP81-cnp3-GFPThis work
MS569h-leu1-32 Sad1-mCherry<<kanR transformed with pREP81-cnp3-1-GFP(S508F)This work
MS615h-leu1-32 GFP-cnp1-hph mis12-mCherry-leu1+This work
MS616h-leu1-32 GFP-cnp1-hph mis12-mCherry-leu1+ cnp3−1This work
MS617h+leu1-32 GFP-cnp1-hph mis12-mCherry-leu1+cnp3−1 mis18-818This work
MS618 *leu1-32 GFP-cnp1-hph mis12-mCherry-leu1+ mis18-818This work
MS620h-leu1-32 GFP-cnp1-hph mis12-mCherry-leu1+ mis18-262This work
MS621leu1-32 GFP-cnp1-hph mis12-mCherry-leu1+ cnp3−1mis18-262This work
TK5h-leu1-32::nmt1GFP-cnp1-leu1+This work
TK85h-leu1-32 ade6-M210 ch16Laboratory stock
  • * : The mating type was not determined.