Table 3 Summary of phenotypes and functions for the different mutant Subito transgenes
Subito mutantDominant effectsRescue of mitotic function (sterility)Rescue of meiotic function (NDJ)Localizes to central spindleRequired for MT organization in oocytesRequired for CPC localization
subitoΔ(1-41)NoNoPartialYesYesYes
subitoΔ(1-21)NoNoNoNoYes-1
subitoΔ(24-33)NoNoYesYesNo
subitoS16AS24AYes (frayed spindles, ectopic)NoYesYes but delocalizedYes, and for restricting Subito to CS 1
subitoΔ(42-76)Yes (spindle pole defects)NoPartialYesYes
subitoΔCT1NoNoNoYesYesYes
subitoΔCT2NoNoNoYesYes
subito3ANoYesYesYesNo
subito3ENoNoNoYesYes
Subito1794NoYes 3No 3Yes 3No 3
  • 1–- = not tested. 2 - CS = Central spindle, 3 - data from (Giunta et al. 2002).