Table 1 Genotypes of strains used
StrainGenotypeComments
JM229caIs85 I; elt-7(tm840) end-1(ok558) end-3(ok1448) V; elt-4(ca16) XcaIs85 I is an integrated multicopy array containing [end-1p::elt-2 cDNA (pJM513) + rol-6 (pRF4) + elt-2p::GFP (pJM370)] and was previously used in (Wiesenfahrt et al. 2016).
JM246caSi2 II; elt-7(+) end-1(ok558) end-3(ok1448) V; elt-4(ca16) X; caEx10caSi2 II is a single copy of an end-1p::elt-2 cDNA construct inserted into the ttTi5605 II site as described by Frøkjaer-Jensen et al. (2012). caEx10 is a non-integrated extrachromosomal multicopy array containing [end-1p::elt-2 cDNA (pJM513) + myo-3p::tdTomato (pJM464) + heatshockpromoter::peel-1 (pMA122)] . JM246 is the starting strain for the current genetic screen.
JM247caSi2 IIcaSi2 II is a single copy insertion of an end-1p::elt-2 cDNA construct into the ttTi5605 II site as described by Frøkjaer-Jensen et al. (2012).
JM274caSi2 II; tasp-1(ca18) III; end-1(ok558) end-3(ok1448) V; elt-4(ca16) X; caEx10
JM279caSi5 I; caSi2 II; caSi6 III; end-1(ok558) end-3(ok1448) V; caEx6Three separate single copy insertions of the end-1p::elt-2 transgene; insertion sites are oxTi185 I, ttTi5605 II, and oxTi444 III (Frøkjaer-Jensen et al. 2012). caEx6 is a non-integrated extrachromosomal multicopy array containing [end-1p::elt-2cDNA (pJM513) + rol-6(pRF4) + elt-2p::GFP(pJM370)]
JM280caSi2 II; pqn-82(gk3768) III; end-1(ok558) end-3(ok1448) V; elt-4(ca16) X
JM281tasp-1(ca18) IIICRISPR/Cas9 induced 29 bp deletion in the tasp-1 gene, as diagrammed on Figure 5A
JM282pqn-82(gk3768) tasp-1(ca18) III
JM283caSi5 I; caSi2 II; caSi6 III;Three separate single copy insertions of the end-1p::elt-2 transgene; insertion sites are oxTi185 I, ttTi5605 II, and oxTi444 III (Frøkjaer-Jensen et al. 2012).
VC3801pqn-82(gk3768) IIICRISPR/Cas9 disruption of the pqn-82 gene