Table 4 Marker alleles present on 2nd chromosome balancers
SM1, SM5, SM6a, CyODuoxCyNonsynonymous C-to-A mutation changing Gly 1505 to Cys, consistent with Hurd et al. (2015).
SM1, SM5, CyOcn2cn2 has two roo insertions: one with a 70-nt target site duplication (TSD) of 2R:7,784,487–7,784,556 that includes the 1st intron and 2nd exon of cn, and the other in the 1st intron of cn with a 6-nt TSD of 2R:7,784,682-7,784,687. Previously reported as an 8-kb insertion in cn (Warren et al. 1996).
SM6a, CyOcn2Pcn2P was induced by EMS treatment (Craymer 1980), and is present on some CyO chromosomes and all SM6a and SM6b chromosomes. Using our dataset, we are unable to identify the difference between cn2 and cn2P.
SM1, SM5, SM6aal2Previously unknown. 10-nt deletion in 4th exon of al resulting in a frameshift. Deletion is from 2L:386,781–386,793. The deletion may be a single deletion or two deletions, as 3 nt of sequence aligns in multiple positions within the 13-nt interval.
SM1, SM5, SM6asp2412 insertion in 5′ UTR of Dat. Previously known (Eric Spana, personal communication).
CyOpr1Previously reported 412 insertion at 2L:20,074,872 (Kim et al. 1996).
SM5ds5521D2–36C inversion breakpoint is in 1st intron of ds. Previously reported (Clark et al. 1995).
SM5ltvPreviously unknown. Doc insertion at/near 2L:22,927,436, which may disrupt the splice acceptor site of the 6th exon. Alternatively, the variegating phenotype may be due to the 40F breakpoint, which may be near lt.
CyOnAChRα6°30F–50D inversion breakpoint is within an intron of nAChRα6. Previously reported (Perry et al. 2007).