Table 2 

G0 and G1 mutagenesis rates in three different Anopheles species.

sgRNA# Inj.SurvivorsMosaic (%)G0M × White FWhite M × G0FG0M × G0F
MFTotal (%)M (%)F (%)G1 Mutant M (%)G1 Mutant F (%)G1 Mutant M (%)G1 Mutant F (%)G1 Mutant M (%)G1 Mutant F (%)
Anopheles coluzziiAcsgRNA16127662138 (23)71 (93)54 (87)991 (93)117 (91)851 (91)1232 (94)881 (86)939 (81)
AcsgRNA2447533689 (20)17 (32)9 (25)1038 (88)1273 (84)751 (89)882 (91)751 (45)846 (49)
Anopheles albimanusAasgRNA15738158139 (24)74 (91)43 (74)N/AN/A1317 (60)1577 (62)
AasgRNA25117968147 (29)00N/AN/AN/AN/A
Anopheles funestusAfsgRNA1237151126 (11)8 (53)7 (64)N/AN/A53 (65)92 (71)
AfsgRNA2352211637 (11)5 (24)4 (25)N/AN/A37 (51)62 (61)