Table 1 Effect of sgRNA and Cas9 concentration on A. coluzzii survival and mutagenesis
AcsgRNA 1Cas9Number InjectedSurvivorsMosaic (%)
MFTotal (%)M (%)F (%)
No injectionNo injection300137118255 (85)00
WaterWater2176952121 (56)00
30 (ng/μl)100 (ng/μl)185313869 (37)32 (46)0
60200251483381 (32)48 (59)0
120300219311647 (21)29 (94)12 (75)
240400177221133 (19)20 (91)9 (82)
480500228121224 (11)12 (100)10 (83)
  • M, male; F, female.