Table 2 Plasmids used in this study
Plasmid No.Plasmid NameDescriptionYeast MarkerEscherichia MarkerRE*SourceAddgene ID
YCplac33-GAL2p-HO-KanMX4pGAL2-HO plasmidKanMX4, URA3AmpN/AHou (et al. 2013)
pRS426Yeast episomal vectorURA3AmpN/AChristianson (et al. 1992)
pXZX010pRS426-GAL2p-HO-KanMX4pGAL2-HO plasmidKanMX4, URA3AmpN/AThis study
pNA0306pRS415-TEF1p-Cas9-CYC1tCas9 expression plasmidLEU2AmpN/ADiCarlo (et al. 2013)#43802
pNA0304pRS426-SNR52p-gRNA-SUP4tBackbone plasmid for gRNA insertionURA3AmpN/ADiCarlo (et al. 2013)#43803
pNA0308pRS426-SNR52p-gRNA-SUP4t-RPR1p-gRNA-RPR1tDouble gRNA backbone plasmidURA3AmpN/AThis study
pXZX339pRS426-SNR52p-gRNA.MATa_option1-SUP4t20-bp gRNA sequence targeting MATa cassetteURA3AmpN/AThis study
pXZX388pRS426-SNR52p-gRNA.MATa_option2-SUP4t20-bp gRNA sequence targeting MATa cassetteURA3AmpN/AThis study
pXZX501pRS426-SNR52p-gRNA.MATa_option3-SUP4t20-bp gRNA sequence targeting MATa cassetteURA3AmpN/AThis study
pXZX351pRS426-SNR52p-gRNA.MATα_option1-SUP4t20-bp gRNA sequence targeting MATα cassetteURA3AmpN/AThis study
pXZX503pRS426-SNR52p-gRNA.MATα_option2-SUP4t20-bp gRNA sequence targeting MATα cassetteURA3AmpN/AThis study
pXZX504pRS426-SNR52p-gRNA.MATα_option3-SUP4t20-bp gRNA sequences targeting MATα cassetteURA3AmpN/AThis study
pXZX352pTOPO-MATaMATa fragment in pTOPO vectorKanNsiIThis study
pXZX353pTOPO-MATαMATα fragment in pTOPO vectorKanNsiIThis study
pXZX448pRS426-SNR52p-gRNA.proto_chr3_L-SUP4t-RPR1p-gRNA-RPR1t20-bp gRNA sequence targeting left end of chromosome IIIURA3AmpN/AThis study
pXZX406pRS426-SNR52p-gRNA.proto_chr3_L-SUP4t-RPR1p-gRNA.proto_chr3_R-RPR1t20-bp gRNA sequences targeting both left and right ends of chromosome IIIURA3AmpN/AThis study
pLM182pGAL1-CEN5-Kl.URA3Kl.URA3AmpNot IReid (et al. 2008)
pLM187pGAL1-CEN10-Kl.URA3Kl.URA3AmpNot IReid (et al. 2008)
  • * RE, restriction enzyme