Table 1 Mapping complementation groups to specific genomic intervals
Complementation groupGenomic interval from deletion mappingComplementing mutationsNoncomplementing mutationsCandidate genesaComments
abb2R:23666959;2371381113
bheGs11, Sam-S1dbrEP9, l(2)gl4Our complementation tests with deletions (Table S4 in File S1) and these mutations indicate bhe1 is a multigene, terminal deletion (Df(2L)bhe) with a breakpoint between dbr and Sam-S. Polytene analysis showed a breakpoint at 21A5-B1. This is consistent with J. Kennison’s observation of at least one bhe allele failing to complement l(2)gl (cited in Lindsley and Zimm 1992). The mutant embryonic phenotype likely results from the disruption of several genes.
blo2R:8733630;8898753RyRQ3878X, snsMI12892, snsMI0300111
bub2L:7702880;77180106
c2R:15918423;1595065210Our data are consistent with unpublished identifications of c as Strn-Mlck by Rodriguez (2004) and E. Spana and E. Green (personal communication).
cass2L:17473293;17482011Aac11k067101These results show cass is the same gene as Aac11.
dw-24E2L:4361214;4403405Tps1k089039Szidonya and Reuter (1988) mapped dw-24E left of Tps1, reducing candidates to five. Curry (1939) showed l(2)cg1 was originally present in the nearby dpy to cl region, but our crosses to spanning deletions showed no lethality, suggesting it was removed before the current stock was established.
eay2R:18173570;1823055411
flz2R:8976399;9031045NpMI00240, NpMI10279CG8213MI046801Our identification of flz as CG8213 is consistent with the independent, unpublished results of Anne Uv (cited in Geberemedhin 2011).
Frd2R:23001651;23068684twi16Our mapping is based on the recessive lethality of Frd1. Frd1 mutants carry an intragenic deletion in PPO3 (Sugumaran and Chase 2004).
fs(2)abc2R:15375176;15386324dupPA771
fs(2)lto32L:19464056;1951761014
fs(2)ltoQE452L:3656901;371382724
fs(2)ltoRM72R:23385467;233959142
hum2R:7395885;7447410soD18Heitzler et al. (1993) mapped hum left of so, reducing candidates to nine.
l(2)21Ba2L:67365;159063Sam-SR23, Sam-S1, Gs1123Caggese et al. (1988) showed l(2)21Ba is not the same gene as Gs1. Larsson et al. (1996) mapped l(2)21Ba right of Sam-S and left of Gs1, reducing candidates to five.
l(2)23Ab2L:2677694;27531259Littleton and Bellen (1994) mapped l(2)23Ab left of Pgk, reducing candidates to seven.
l(2)24Dc2L:4162968;41978005
l(2)24Dd2L:4031318;4162968ed15Szidonya and Reuter (1988) showed l(2)24Dc is not the same gene as ed.
l(2)24De2L:4162968;41978005
l(2)25Cg2L:5073453;51455005Szidonya and Reuter (1988) showed l(2)25Cg is not the same gene as Msp300, reducing candidates to four.
l(2)34Db2L:13721648;1380082916John Roote reported l(2)34Db maps right of P{lacW}TM9SF4k07245 (http://flybase.org/reports/FBrf0129261.html) and left of Df(2L)Exel7059 (personal communication), reducing candidates to nine. This is consistent with the tentative conclusion that l(2)34Db is the same gene as Sec71 (Ashburner et al. 1999).
l(2)35De2L:15821840;1591234315Our data maps l(2)35De to one of two intervals, but this interval is consistent with the mapping of Ashburner et al. (1999).
l(2)36Ba2L:16824908;1688655714
l(2)36Fe2L:18606977;186172253
l(2)37Ab2L:18673286;18689053msl-1kmB4
l(2)37Ac2L:18753432..18753444;187958202
l(2)37Dc2L:19438065;194529184
l(2)37De2L:19438065;194529184
l(2)37Di2L:19464056;19517610swm37Dh-113Brittnacher and Ganetzky (1983) showed l(2)37Di is not the same gene as swm.
l(2)37Ea2L:19517610;195283835
l(2)37Fb2L:19576108..19576133;195863756Rutledge et al. (1992) and Gay and Contamine (1993) showed l(2)37Fb is not the same gene as spi, reducing candidates to five.
l(2)37Fc2L:19576108..19576133;195863756Rutledge et al. (1992) and Gay and Contamine (1993) showed l(2)37Fc is not the same gene as spi, reducing candidates to five.
l(2)37Fe2L:19586375;19753324Lar13.27Butler et al. (2001) showed l(2)37Fe is not the same gene as scw, reducing candidates to six.
l(2)38Ab2L:20085397;20120504nebk057027
l(2)38Db2L:20449190..20458307;2063858024–26
l(2)38Eb2L:20680624;20770538Hr3802306, Fs(2)KetRX311Butler et al. (2001) and Kozlova et al. (2009) showed l(2)38Eb is not the same gene as dia, reducing candidates to 10.
l(2)38EFb2L:20831386;208519006
l(2)43Ba2R:7187225;73269519Heitzler et al. (1993) showed l(2)43Ba is not the same gene as pwn, reducing candidates to eight.
l(2)43Cc2R:7493197;7533553dpaEY04015, dpa111Heitzler et al. (1993) mapped l(2)43Cc right of dpa, reducing candidates to nine. MacIver et al. (1998) tentatively identified l(2)43Cc as didum.
l(2)43Da2R:7493197;7533553dpa1, dpaEY0401512Heitzler et al. (1993) mapped l(2)43Da right of dpa, reducing candidates to 10.
l(2)43Db2R:7493197;7533553dpaEY0401512Heitzler et al. (1993) mapped l(2)43Db right of dpa, reducing candidates to 10.
l(2)43Ef2R:7665795;7708707tor4, U2A19Heitzler et al. (1993) showed l(2)43Ef is not the same gene as U2A or tor and maps right of U2A. Nagengast and Salz (2001) showed a U2A transgene did not rescue l(2)43Ef mutations. This reduces candidates to four.
l(2)43Eg2R:7665795;7708707U2A110Heitzler et al. (1993) showed l(2)43Eg maps right of U2A and is not the same gene as tor, reducing candidates to four.
l(2)46Ca2R:9875312;9922003..9927457tea1755Etf-QOf056401These results show l(2)46Ca is the same gene as Etf-QO.
l(2)46Cb2R:9875312;9922003..9927457Etf-QOf05640, tea17558O’Brien et al. (1994) showed l(2)46Cb is not the same gene as FMRFa, reducing candidates to seven.
l(2)46Cd2R:9958120;10025288eve3, eve5, Pal1ta-1, eIF3jk139069
l(2)46Db2R:9959818;10025288eve3, eve5, Pal1ta-1, eIF3jk13906, TER94k15502, TER94EY03486TER9403775, TER9426-8, TER9422-301These results show l(2)46Db is the same gene as TER94, even though l(2)46Db26 shows a complex complementation pattern with other TER94 alleles.
l(2)46Dc2R:10025288;100305393
l(2)46Dd2R:10030539;1007829316
l(2)46De2R:9959818;10025288..10025310eve5, eIF3jk139068O’Brien et al. (1994) mapped l(2)43De right of eve, reducing candidates to four.
l(2)46Df2R:10030539;1007829316
l(2)49Dc2R:12894105..12894116;12940453..1294905512–13
l(2)49Fa2R:13197974..13198492;13219347..1321934910Lasko and Pardue (1988) showed l(2)49Fa is not the same gene as Orc3, reducing candidates to nine.
l(2)49Fg2R:13219130;1324924114Lasko and Pardue (1988) mapped l(2)49Fg left of Dp, reducing candidates to three.
l(2)51Ea2R:15218008;15262942scb21These results show l(2)51Ea is the same gene as scb.
l(2)57Ba2R:21000163;210567988
l(2)57Bd2R:21056798;2108824712
l(2)57Cb2R:21056798;2108824712
l(2)57Cc2R:21143577;2117731012J. M. O’Donnell et al. (1989) suggested l(2)57Cc is adjacent to Pu or overlaps it. Reynaud et al. (1999) suggested l(2)57Cc is not the same gene as Xpd.
l(2)57Cd2R:21143577;2117731012J. M. O’Donnell et al. (1989) suggested l(2)57Cd is adjacent to Pu or overlaps it. Reynaud et al. (1999) suggested l(2)57Cd is not the same gene as Xpd.
l(2)57Ce2R:21180990;212152239J. O’Donnell et al. (1989) showed l(2)57Ce is not the same as tud. This reduces candidates to eight.
l(2)57Db2R:21301798;2134164723
l(2)57Eb2R:21497209;2160708116J. O’Donnell et al. (1989), Price et al. (1989), and Schejter and Shilo (1989) showed l(2)57Eb is not the same gene as Egfr, reducing candidates to 15.
l(2)57Ec2R:21497209;2160708116J. O’Donnell et al. (1989) showed l(2)57Ec is not the same gene as Egfr, reducing candidates to 15.
l(2)DA22L:9522946;95604897
l(2)DB22L:9897536;99084592
l(2)DB42L:9205076;938812920Lane and Kalderon (1993) showed l(2)DB4 is not the same gene as Cks30A, reducing candidates to 19.
l(2)FE3hoipk071041These results show l(2)FE3 is the same gene as hoip. It was mapped with cytologically defined deletions (Table S4 in File S1), but not molecularly defined deletions, so no genomic interval is given.
l(2)N7-62L:9622987;96992255
l(2)N7-82L:9205076;9388129Cks30ARA7419Lane and Kalderon (1993) showed l(2)N7-8 is not the same gene as Cks30A.
l(2)PC4-A2R:18051197;18118348stau111
l(2)PC4-D2R:17782032;177926494Mohr and Gelbart (2002) mapped l(2)PC4-D to Ubc10, CG5033 or Dhit, reducing candidates to two.
l(2)PC4-M2R:17716263..17716471;17739901..1773991611
l(2)PC4-P2R:18621522;186392686
l(2)PC4-Q2R:18621522;186392686
mat(2)syn-E2L:10349604;1038121411Our data place mat(2)syn-E in the same general region as Clegg et al. (1993), but they placed it right of da and RpS27A.
moa2R:22729367;227649353
ms(2)35Eb2L:15912343;1602536916
nrd2L:2517598..2551864;262101616–19Littleton and Bellen (1994) mapped nrd right of Drp1, reducing candidates to five. Our results are compatible with nrdD20 being associated with a small deletion as proposed by Littleton and Bellen.
P{f+13}30B2L:9205076;938812920These crosses show a lethal mutation (hereafter l(2)30ABa1) is caused by the P{f+13}30B insertion or is closely linked to it.
pd2R:23811400;23844351shu27
qui2R:23659139;2371381112
sat2R:7493197;7533553Orc1KO1These results show sat is the same gene as Orc1.
sie2R:13034847;1315957910
• a Excludes genes with complementing mutations from the set of contiguous genes defined by deletion breakpoints (Table S3 in File S1). Ranges reflect deletion breakpoint uncertainty. Candidate genes are listed in Table S4 in File S1.