Table 2 Polymorphisms detected in glycinin and β-conglycinin genes in PI 603408 as compared to the Williams 82 a2.v1 genome
FamilyWm82.a2.v1Wm81.a1.v1.1Gene NameSeed Protein(s)Variants IdentifiedAmino Acid ChangeSIFT PredictionSIFT ScoreSeed Expression
β-conglycininGlyma.10g028300Glyma10g03390CG-1CG-α′-subunit149C > APro50GlnTolerated1Seed expressed
Glyma.10g028300Glyma10g03390CG-1CG-α′-subunit192A > TArg64SerTolerated0.654Seed expressed
Glyma.10g246300Glyma10g39150CG-1CG-α′-subunit1280G > AArg427HisTolerated0.223Seed expressed
No correspondenceGlyma10g39161NANo correspondence
Glyma.10g246500Glyma10g39170NANA
Glyma.20g146200Glyma20g28460CG-4CG-β-subunit108G > TLeu36PheTolerated1Seed expressed
Glyma.20g148200Glyma20g28640CG-4CG-β-subunit
Glyma.20g148400Glyma20g28650CG-2CG-α-subunitSeed expressed
Glyma.20g148300Glyma20g28660CG-3CG-α-subunitSeed expressed
GlycininNo correspondenceGlyma03g32010Gy6aaNANo correspondencePseudogene
No correspondenceGlyma03g32020Gy2A2 B2No correspondenceSeed expressed
Glyma.03g163500Glyma03g32030Gy1A1a B1a604_606delCATinsTCCHis202SerTolerated0.74Seed expressed
Glyma.03g163500Glyma03g32030Gy1A1a B1a151C > TLeu51PheTolerated0.229Seed expressed
Glyma.10g037100Glyma10g04280Gy4A4 A5 B3Seed expressed
Glyma.13g123500Glyma13g18450Gy5A3 B4584_585delTAIle195fsNAbNAbSeed expressed
Glyma.19g164800Glyma19g34770Gy7aNA1031T > AVal344GluTolerated0.951Pseudogene
Glyma.19g164800Glyma19g34770Gy7aNA910G > AAsp304AsnTolerated0.085Pseudogene
Glyma.19g164900Glyma19g34780Gy3A1b B1bSeed expressed
  • SIFT, Sorting Intolerant From Tolerated; NA, not applicable; 4G, four genomes.

  • a Pseudogene.

  • b SIFT 4G does not interpret multiple nucleotide polymorphisms.