Table 2 Assembly statistics before and after rescaffolding with long read clouds using fragScaff
Original Assembly (with N’s)Original Assembly (No N’s)Rescaffolded Assembly (with N’s)Rescaffolded Assembly (no N’s)
Maximum scaffold length (bp)4,064,3363,809,09623,976,85122,367,058
N50324,201306,8972,668,3662,509,905
N10959,930904,5018,710,9938,182,563
N9072,46069,664406,554399,889
  • The weighted average assembly length (N50) increased by ∼ eightfold. This was regardless of whether or not padding (N’s) was included in the calculation.