Table 6 SD/aSD binding of nonhypothetical genes in B. subtilis phage φ29 in E. coli and B. subtilis
GeneE. coliB. subtilis
DtoStartaSDDtoStartbSD
gp214AAGGA17AAAGGA
gp317AAGGAG20GAAAGGAG
gp418AGGAGGU21AGGAGGU
gp515AAGGA18AAAGGA
gp619UAGAAAG
gp716GAGGUGA18,19UAGAAAG,GAGGUGA
gp818GAGGU21,21AGAAA,GAGGU
gp8.520GGAGGUG23GGAGGUG
gp916,19UAAGG,AGGUG22AGGUG
gp1015GAGGUGA18GAGGUGA
gp1116GGUGA19GGUGA
gp1215UAAGGAGG18AAGGAGG
gp1317GAGGU20GAGGU
gp1417AAGGAG20AAAGGAG
gp1517UAAGGAGG20AAGGAGG
gp1616GAGGUG19GAGGUG
  • Gene gp6, which uses a species-specific SDBs, cannot form a well-positioned SD/aSD in E. coli to be translated efficiently.

  • a The optimal DtoStart is within the range of 10–21 in E. coli.

  • b 3′AUCUUUCCUCCACUAG is used as 3′TAIL for B. subtilis, with the optimal DtoStart within the range of 15–25.