Table 4 Primers to amplify target fragments
Sequence (5′–3′)LengthTm (°C)GC (%)
Forward primerCAGATAGCCAGCACTTAGCCC216157.14
Reverse primerCCTGTTGTGAGTGGAGTGTGG216157.14
Product length746
  • PCR amplification in a volume of 40 μl, containing 20 μl PCR Mix (TaKaRa), 50 ng template, 0.5 μmol/l of each primer. The PCR conditions were: 95° for 3 min, followed by 36 cycles of 94° for 30 sec, 62° for 30 sec, 72° for 1 min, and a final extension step at 72° for 5 min.