Table 1 List of strains used in this work
StrainParentDescriptionReference
KUE1002001HParent strain, histidine auxotroph, the recipient enable high efficient gene targeting in which yku80 is repressed with a SAT1 flipperUeno et al. (2011)
KUE100_ΔCgBag7KUE100ΔCgBag7 strain, CgBAG7 gene (ORF CAGL0I07249g) was replaced with CgHIS3 markerThis study
KUE100_ΔCgCmr3KUE100ΔCgCmr3 strain, CgCMR3 gene (ORF CAGL0L05786g) was replaced with CgHIS3 markerThis study
KUE100_ΔCgEno2KUE100ΔCgEno2 strain, CgENO2 gene (ORF CAGL0F08261g) was replaced with CgHIS3 markerThis study
KUE100_ΔCgFps1KUE100ΔCgFps1 strain, CgFPS1 gene (ORF CAGL0C03267g) was replaced with CgHIS3 markerThis study
KUE100_ΔCgFps2KUE100ΔCgFps2 strain, CgFPS2 (Acs# CAGL0E03894g) was replaced with CgHIS3 markerThis study
KUE100_ΔCgGad1KUE100ΔCgGad1 strain, CgGAD1 gene (ORF CAGL0H02585g) was replaced with CgHIS3 markerThis study
KUE100_ΔCgHaa1KUE100ΔCgHaa1 strain, CgHAA1 gene (ORF CAGL0L09339g) was replaced with CgHIS3 markerThis study
KUE100_ΔCgHsp30KUE100ΔCgHsp30 strain, CgHSP30 gene (ORF CAGL0K07337g) was replaced with CgHIS3 markerThis study
KUE100_ΔCgMit1KUE100ΔCgMit1 strain, CgMIT1 gene (ORF CAGL0C03740g) was replaced with CgHIS3 markerThis study
KUE100_ΔCgRsb1KUE100ΔCgRsb1 strain, CgRSB1 gene (ORF CAGL0L10142g) was replaced with CgHIS3 markerThis study
KUE100_ΔCgSsa3KUE100ΔCgSsa3 strain, CgSSA3 gene (ORF CAGL0G03289g) was replaced with CgHIS3 markerThis study
KUE100_ΔCgSut2KUE100ΔCgSut2 strain, CgSUT2 gene (ORF CAGL0I04246g) was replaced with CgHIS3 markerThis study
KUE100_ΔCgTpo3KUE100ΔCgTpo3 strain, CgTPO3 gene (ORF CAGL0I10384g) was replaced with CgHIS3 markerCosta et al. (2014)
KUE100_ΔCgYps4KUE100ΔCgYps4 strain, CgYPS4 gene (ORF CAGL0E01749g) was replaced with CgHIS3 markerThis study
KUE100_ΔCAGL0E03740gKUE100ΔCAGL0E03740g strain, ORF CAGL0E03740g was replaced with CgHIS3 markerThis study
KUE100_ΔCAGL0G05632gKUE100ΔCAGL0G05632g strain, ORF CAGL0G05632g was replaced with CgHIS3 markerThis study
ATCC2011ATCC2001Parent strain
ATCC2001_ ΔCgHaa1ATCC2001ΔCgHaa1 strain, CgHAA1 (ORF CAGL0L09339g) was replaced by the norseotruchin resistance SAT1 flipper cassetteScwarzmuller et al. (2014)