Table 3 C glabrata genes whose acetic acid-induced transcriptional activation was partly mediated by CgHaa1
ORFC. glabrata GeneLogFC (wt AC/wt CTRL)LogFC (ΔCghaa1AC/ΔCghaa1 CTRL)FunctionS. cerevisiae OrthologS. cerevisiae Ortholog Regulated by ScHaa1?S. cerevisiae Homolog Confers Resistance to Acetic Acid?
CAGL0I06182gCgPIR27,653,10O-mannosylated heat shock protein that is secreted and covalently attached to the cell wall via β-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitationHSP150Not describedNo
CAGL0G05632g6,951,41Ortholog(s) have cytoplasm localizationYDL218WNot describedNo
CAGL0I10010g6,823,66v-SNARE binding protein that facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H+-ATPaseBTN2Not describedNo
CAGL0M01166g6,444,20Ortholog(s) have ferrous iron binding activity, role in mitochondrial genome maintenance, thiamine biosynthetic process, thiazole biosynthetic process, and cytosol or nucleus localizationTHI4Not describedNo
CAGL0F08261g6,112,55Ortholog(s) have phosphopyruvate hydratase activity, role in glycolysis, regulation of vacuole fusion, nonautophagic and fungal-type vacuole, internal side of plasma membrane, mitochondrion, and phosphopyruvate hydratase complex localizationENO1Not described
CAGL0G03883g6,104,05Disaggregase; Heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagationHSP104Yes
CAGL0G03289gCgSSA36,102,09Heat shock protein of the HSP70 familySSA4YesYes
CAGL0J00451g5,664,38Putative glyceraldehyde-3-phosphate dehydrogenase; protein differentially expressed in azole resistant strain; expression downregulated in biofilm vs. planktonic cell cultureTDH3Not describedNo
CAGL0M08822gCgHSP785,063,34Ortholog(s) have ATPase activity, misfolded protein binding activityHSP78Not describedNo
CAGL0E00803g4,822,88Putative small cytosolic stress-induced chaperone; gene is upregulated in azole-resistant strainHSP42YesNo
CAGL0H03707g4,642,57Ortholog(s) have role in protein folding, translational initiation and cytosolic small ribosomal subunit, nucleus localization*SIS1Not describedNo
CAGL0G08866g4,381,96Ortholog(s) have RNA polymerase II transcription factor binding, RNA polymerase II transcription factor binding transcription factor activity, sequence-specific DNA binding activityFKH2YesNo
CAGL0C02321gCgPHM84,252,36Ortholog(s) have nucleotidase activity and role in pyrimidine nucleobase metabolic processSDT1Not describedNo
CAGL0K10164g4,082,54Predicted GPI-linked protein; putative adhesin-like proteinSPI1YesNo
CAGL0I09724g4,072,05UnknownNo
CAGL0F04631g4,031,84No description availableMOH1Not describedNo
CAGL0F04631g4,031,84Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phaseMOH1Not describedNo
CAGL0I10384gCgTPO33,981,49Predicted polyamine transporter of the major facilitator superfamily; required for azole resistanceTPO3YesYes
CAGL0J06050g3,971,41Has domain(s) with predicted role in cellular amino acid metabolic processYGP1YesNo
CAGL0H00704g3,952,86Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrateICY2Not describedYes
CAGL0G08624gCgQDR23,922,69Drug:H+ antiporter of the Major Facilitator Superfamily, confers imidazole drug resistance, involved in quinidine/multidrug efflux; gene is activated by Pdr1p; upregulated in azole-resistant strainQDR1Not describedNo
  • The genes found to be upregulated in response to acetic acid in the wild-type and in the ΔCghaa1 strains were compared and those genes whose acid-induced activation was reduced by more than 50% in the mutant background were selected as CgHaa1 targets. A subset of these CgHaa1-activated genes is shown in this table but the full list is shown in Table S3. The biological function indicated was based on the information available at the Candida Genome Database website or on the information available for the corresponding S. cerevisiae ortholog. Essential genes are indicated with * (predicted based on the information available for S. cerevisiae). Information on the involvement of the corresponding S. cerevisiae (Sc) orthologs in tolerance to acetic acid and their inclusion in the ScHaa1-dependent transcriptional regulatory network is also shown, based on the information available in the YEASTRACT database (Teixeira et al. 2014) or on published results (Mira et al. 2010a,b). ORF, open reading frame; AC, acetic acid; CTRL, Control; SNARE, soluble NSF attachment protein receptor; ATPase, adenosine triphosphatase; GPI, glycosylphosphatidylinositol. log-log2