Table 1 ITS2 sequence information for individual specimens
NGSSanger
VAITCSpecies NameNo. of AllelesSize Range (bp)Average Size (bp)Bases > Q20 (%)Post-Trim (bp)Coverage of NGS (%)
4333Aedeomyia venustipes152252294.045386.8
4560AAedeomyia venustipes2509–52251693.445488.1
4560BAedeomyia venustipes152252291.845086.2
4309AAnopheles annulipes2336–35734791.0518149.5a
4309BAnopheles annulipes2282–31629990.2520173.9a
4309CAnopheles annulipes130530591.3523171.5a
4315ACoquillettidia linealis4454–48546890.141388.3
4315BCoquillettidia linealis148248284.838078.8
4332BCoquillettidia linealis148348383.238078.7
4342Culex annulirostris144244280.932373.1
4344Culex annulirostris2444–44644570.325156.4
4345Culex annulirostris3442–44744467.125156.6
4314BCulex annulirostris2440–44744478.932874.0
4324BCulex annulirostris4438–44944280.527762.6
4310ACulex australicus2476–48247984.636476.0
4310BCulex australicus3470–47847485.337078.0
4326ACulex australicus147847836.912626.4
4326BCulex australicus3478–48648287.439181.1
4326CCulex australicus2478–48248062.812425.8
4348BCulex cylindricus5378–43241092.636689.3
4348CCulex cylindricus4394–43240892.937090.7
4348DCulex cylindricus7394–43241891.236186.3
4348ECulex cylindricus2396–43241491.936487.9
4682BCulex palpalis3436–44644171.323853.9
4683ACulex palpalis2442–44944655.310724.0
4312ACulex pipiens form molestus2501–51250733.39919.5
4312BCulex pipiens form molestus2499–50850463.719638.9
4339ACulex pipiens form molestus150650693.044688.1
4339BCulex pipiens form molestus150150134.110420.8
4318BCulex quinquefasciatus3492–50449740.89519.1
4318CCulex quinquefasciatus150150167.429959.7
4318DCulex quinquefasciatus150050034.911122.2
4318ECulex quinquefasciatus149949992.243787.6
4903Culiseta inconspicua139339389.532181.7
4904Culiseta inconspicua139339389.832281.9
4329BDobrotworskyius alboannulatus17376–39939184.329575.5
4331ADobrotworskyius alboannulatus11376–39739088.431981.7
4331BDobrotworskyius alboannulatus12376–39939289.832783.5
4331CDobrotworskyius alboannulatus13376–39939181.230778.5
4681Dobrotworskyius rubrithorax24318–40437962.617245.4
4902Dobrotworskyius rubrithorax35318–40338460.117545.5
4327ADobrotworskyius rubrithorax31319–40137862.317947.3
4327BDobrotworskyius rubrithorax24341–40438658.715439.9
4551Macleaya macmillani2433–43443490.736283.5
4334Macleaya tremula241041088.733782.2
4556Macleaya tremula2411–43342273.430973.2
4905Macleaya tremula141041090.134283.4
4335Macleaya wattensis3418–42041979.930171.8
4558Macleaya wattensis2416–42642188.334782.4
4323Mucidus alternans139239258.921655.1
4328AMucidus alternans2392–39639459.419349.0
4328BMucidus alternans2392–39639478.228572.3
4336AMucidus alternans3385–39439082.928773.5
4336BMucidus alternans3356–39638177.327672.4
4308AOchlerotatus bancroftianus2480–48248182.036575.9
4308BOchlerotatus bancroftianus3480–49148490.040884.4
4308COchlerotatus bancroftianus4475–49148347.613026.9
4308DOchlerotatus bancroftianus4478–49148588.139781.9
4330Ochlerotatus camptorhynchus2383–38738588.432784.9
4341Ochlerotatus camptorhynchus3372–38737889.932485.8
4347Ochlerotatus camptorhynchus3368–38737890.131783.8
4304BOchlerotatus camptorhynchus139139183.730277.2
4303Ochlerotatus mallochi5418–43742889.037086.4
4325Ochlerotatus mallochi2425–43643173.626260.9
4553Ochlerotatus mallochi2425–43643189.235983.4
4554Ochlerotatus mallochi5425–43643089.937887.8
4555Ochlerotatus mallochi5425–43643086.035682.7
4305AOchlerotatus sagax3401–40740365.421052.1
4305BOchlerotatus sagax4400–41140665.221352.5
4305COchlerotatus sagax5388–40740078.028671.6
4305DOchlerotatus sagax5400–41040566.220951.6
4302AOchlerotatus theobaldi2423–42542479.128667.5
4302BOchlerotatus theobaldi3411–42841978.431073.9
4302COchlerotatus theobaldi3419–42842380.929269.0
4302DOchlerotatus theobaldi2419–42642381.930271.5
4301AOchlerotatus vittiger2406–41040889.733782.6
4301BOchlerotatus vittiger2406–40840780.828670.3
4301COchlerotatus vittiger4406–41441080.128268.9
4301DOchlerotatus vittiger2406–41441070.021251.7
4319ARampamyia notoscripta5426–46644087.435480.4
4319BRampamyia notoscripta7365–46442750.712328.8
4319CRampamyia notoscripta7351–46442381.337388.2
4337ARampamyia notoscripta6339–43441282.333280.6
4337BRampamyia notoscripta6412–46443584.433677.3
4313Tripteroides atripes2356–35935886.827376.4
4320Tripteroides atripes2356–35935884.426072.7
4316ATripteroides sp.3354–36436080.824066.7
4349Tripteroides tasmaniensis137637683.728776.3
  • NGS, next-generation sequencing; VAITC, Victorian Agricultural Insect Tissue Collection; No., number.

  • a See text regarding NGS limitation of resolving full length An. annulipes alleles.