Table 4 Genomic edits confirming an MMP allele and modeling a human allelic series at the conserved residue INSR(A1135)
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  • CRISPR-Cas9 was used to alter DAF-2(A1391) to the indicated residues and the results are shown for dauer assays performed at indicated temperatures. As observed with human INSR(A1135) mutations, these alteration result in a graded range of phenotypes, from no observable phenotype to constitutive dauer formation at all tested temperatures. N.D., not determined. Numbering refers to the preproreceptor. Color as described in Figure 3.