Table 2 C. glabrata genes whose acetic acid-induced transcriptional activation was fully mediated by CgHaa1
ORFC. glabrata GeneLogFC (wt AC/wt CTRL)FunctionS. cerevisiae OrthologS. cerevisiae Ortholog Regulated by ScHaa1?S. cerevisiae Homolog Confers Resistance to Acetic Acid?
CAGL0C04323g5,33Ortholog(s) have α,α-trehalase activity, role in trehalose catabolic process, and cytoplasm localizationNTH1Not describedNo
CAGL0I07249g4,71Putative GTPase-activating protein involved in cell wall and cytoskeleton homeostasis; gene is upregulated in azole-resistant strainBAG7Not describedNo
CAGL0K07337g4,37Has domain(s) with predicted ion channel activity, role in ion transport, and membrane localizationHSP30YesNo
CAGL0H02585g4,19Ortholog(s) have glutamate decarboxylase activity, role in cellular response to oxidative stress, glutamate catabolic process, and cytoplasm localizationGAD1YesNo
CAGL0H10076g4,12Has domain(s) with predicted ion channel activity, role in ion transport, and membrane localizationYRO2YesYes
CAGL0G06182g3,72No description availableYHR131CNot describedNo
CAGL0A01804gCgHXT13,65Ortholog(s) have fructose transmembrane transporter activity, pentose transmembrane transporter activity, role in glucose transport, mannose transport, and plasma membrane localizationHXT1Not describedNo
CAGL0K03421g3,39Ortholog(s) have cytosol, nucleus localizationPGM2YesNo
CAGL0L08008g3,39No description availablePMP2Not describedYes
CAGL0G05269g3,27Putative mitochondrial protein; gene is downregulated in azole-resistant strainFMP16Not describedNo
CAGL0I09702g3,24Ortholog(s) have riboflavin transporter activity, role in riboflavin transport, and plasma membrane localizationMCH5YesYes
CAGL0E05148g3,23Ortholog(s) have α-mannosidase activity, role in oligosaccharide catabolic process, and cytosol, fungal-type vacuole membrane localizationAMS1Not describedNo
CAGL0G02057g3,19Ortholog(s) have cytoplasm, nucleus localizationYKR075CYesNo
CAGL0H04851g3,18Ortholog(s) have 4-nitrophenylphosphatase activity, protein serine/threonine phosphatase activity, and role in cellular protein localization, cellular sodium ion homeostasis, and protein dephosphorylationPPZ1Not describedNo
CAGL0A02002g3,16No description availableYOL024WNot describedNo
CAGL0K07590gCgMYO33,11Putative myosinMYO3Not describedNo
CAGL0I05148gCgDLD13,07D-lactate ferricytochrome C oxidoreductaseDLD1Not describedNo
CAGL0G02563g3,06Has domain(s) with predicted ubiquitin thiolesterase activity and role in ubiquitin-dependent protein catabolic process
CAGL0G03179g3,06Has domain(s) with predicted phospholipid binding activity*ASK10Not describedNot tested
CAGL0A00495gCgPMA12,98Putative plasma membrane proton pump with a predicted role in pH homeostasis*PMA1Not describedNot tested
CAGL0A01870g2,97Has domain(s) with predicted integral to membrane localization
CAGL0I06644g2,94Putative GPI-linked cell wall proteinSPI1Not describedNo
CAGL0M06897g2,91Ortholog(s) have cytoplasm localizationYNL024CYesNo
CAGL0H07469g2,90Putative adhesin-like proteinICS2Not describedNo
CAGL0G05698gCgGDH22,89Ortholog(s) have glutamate dehydrogenase (NAD+) activity, role in nitrogen compound metabolic process, and cytosol, mitochondrion localizationGDH2Not describedNo
CAGL0A01716g2,87Ortholog(s) have nicotinamidase activity, role in chromatin silencing at rDNA, chromatin silencing at telomere, replicative cell aging, and cytosol, nucleus, and peroxisome localizationPNC1Not describedNo
CAGL0E03630g2,84Ortholog(s) have RNA binding activity and role in negative regulation of conjugation with cellular fusion, premeiotic DNA replication, reciprocal meiotic recombination, and sporulation resulting in formation of a cellular sporeRIM4Not describedNo
CAGL0J11462g2,84Predicted GPI-linked cell wall proteinYNL190WNot describedNo
CAGL0G03267g2,80Ortholog(s) have role in protein targeting to membrane and cytoplasm localizationAST2Not describedNo
CAGL0A01650g2,80Putative protein; gene is upregulated in azole-resistant strainECL1YesNo
CAGL0E01749gCgYPS42,78Putative aspartic protease; member of a YPS gene cluster that is required for virulence in mice; induced in response to low pH and high temperatureYPS1Not describedNo
  • The genes found to be upregulated in response to acetic acid in the wild-type and in the ΔCghaa1 strains were compared and those genes whose acid-induced activation was abrogated in the mutant background were selected as CgHaa1 targets. A subset of these CgHaa1-activated genes is shown in this table but the full list is shown in Table S3. The biological function indicated was based on the information available at the Candida Genome Database website or on the information available for the corresponding S. cerevisiae ortholog. Essential genes are indicated with * (predicted based on the information available for S. cerevisiae). Information on the involvement of the corresponding S. cerevisiae (Sc) orthologs in tolerance to acetic acid and their inclusion in the ScHaa1-dependent transcriptional regulatory network is also shown, based on the information available in the YEASTRACT database (Teixeira et al. 2014) or on published results (Mira et al. 2010a,b). ORF, open reading frame; AC, Acetic acid; CTRL, Control; GTPase, guanosine triphosphatase; GPI, glycosylphosphatidylinositol; NAD, nicotinamide, adenine dinucleotide; YPS, Yersinia pseudotuberculosis. log - log2