Table 2 Activity rhythms of strains used to validate candidate clock phosphatases
Genotypen% RhythmicPeriod Mean ± SEM
w1118;+/GD351714310023.73 ± 0.04
w1118;timGal4/GD35171169424.43 ± 0.08
w1118;GD35171/+;timGal4/+119124.50 ± 0.19
w1118;GD35171/+;pdfGal4/+159323.82 ± 0.07
w1118;;UAS-mts/+1210023.5
w1118;timGal4/+;UAS-mts/+80AR
w1118;;UAS-mts/pdfGal4144323.30 ± 0.18
w1118;P{EP}Pp2A-29BEP2332/+1610023.63 ± 0.05
w1118;P{EP}Pp2A-29BEP2332/timGal41510023.90 ± 0.13
w1118;P{RS3}Pp2A-29BCB-5426-3168823.57 ± 0.05
w1118;PBac{WH}CG17746f05041119123.30 ± 0.08
y1w*;P{EP}CG17746G4827169423.53 ± 0.06
y1w1118;PBac{3HPy+}I-2C3621410023.75 ± 0.13
w*;P{UAS-I-2.HA}G/+;P{UAS-Pp1-87B.HA}1H/+1510023.67 ± 0.11
w*;P{UAS-I-2.HA}G/timGal4;P{UAS-Pp1-87B.HA}1H/+158724.15 ± 0.15
w*;P{UAS-I-2.HA}G/+;P{UAS-Pp1-87B.HA}1H/pdfGal4910024.22 ± 0.11
y1w67c23;P{SUPor-P}CG7115KG02655127523.50 ± 0.14
w1118;UAS-Cep97/+1510024.27 ± 0.14
w1118;UAS-Cep97/timGal4910024.28 ± 0.08
w1118;UAS-Cep97/+;pdfGal4/+1310024.67 ± 0.54
UAS-Cep97/Y149323.38 ± 0.06
UAS-Cep97/Y;timGal4/+156023.83 ± 0.11
UAS-Cep97/Y;;pdfGal4/+138523.59 ± 0.06
w1118;;UAS-Cep97/+1510023.5
w1118;timGal4/+;UAS-Cep97/+1610023.97 ± 0.10
w1118;;UAS-Cep97/pdfGal41610024.31 ± 0.10
y1;P{SUPor-P}tocKG08989PpD6KG089891110023.68 ± 0.12
y1w*Mi{MIC}Ptpmeg2MI03011/Y1510023.73 ± 0.08
w67c23P{lacW}Ptpmeg2G0232/Y166324.65 ± 0.21
y1w67c23P{Mae-UAS.6.11}Ptpmeg2GG01129/Y169423.57 ± 0.07
y1w67c23P{Mae-UAS.6.11}Ptpmeg2GG01129/Y;timGal4/+109024.22 ± 0.19
y1w67c23P{Mae-UAS.6.11}Ptpmeg2GG01129/Y;;pdfGal4/+1510023.80 ± 0.09
w1118PBac{WH}Ptpmeg2f06600/Y1310023.62 ± 0.11
w*;;Ptp69D188826.57 ± 0.21
w*;;Df(3L)8ex25106026.5
w1118;;Ptp69D10159326.96 ± 0.14
w1118;;Ptp69D18165027.19 ± 0.15
w1118;;Ptp69D201010023.60 ± 0.06
w1118;;Ptp69D211610023.53 ± 0.30
w1118;;UAS-Ptp69D/+98924.19 ± 0.22
w1118;;UAS-Ptp69D/pdfGal4126724.13 ± 0.19
w1118;;timGal4/+;UAS-Ptp69D/+129227.82 ± 0.28
w1118;;UAS-DNPtp69D/+1510023.47 ± 0.09
w1118;;UAS-DNPtp69D/pdfGal486327.20 ± 0.18
w1118;;timGal4/+;UAS-DNPtp69D/+1510023.50 ± 0.05
w1118;;Ptp69D1iso810023.44 ± 0.06
y1P{SUPor-P}MKP-4KG034201310024.23 ± 0.12
w1118;;P{GSV6}Pp1α-96AGS11179/+1610023.59 ± 0.08
w1118;timGal4/+;P{GSV6}Pp1α-96AGS11179/+162524.13 ± 0.21
w1118;;P{GSV6}Pp1α-96AGS11179/pdfGal496723.67 ± 0.15
w1118;;Pp1α-96A2/+169423.77 ± 0.12
w1118;;UAS-Pp1α-96A.HA/+1610023.38 ± 0.05
w1118;timGal4/+;UAS-Pp1α-96A.HA/+169423.87 ± 0.11
w1118;;UAS-Pp1α-96A.HA/pdfGal4169423.33 ± 0.08
w1118;;Pp1α-96A-CRISPRmutant-1/+178223.52 ± 0.07
w1118;;Pp1α-96A-CRISPRmutant-2/+139223.58 ± 0.05
w1118;;Pp1α-96A-CRISPRmutant-3/+910023.44 ± 0.05
w1118;UAS-CG10417/+1610023.44 ± 0.04
w1118;UAS-CG10417/timGal41410024.96 ± 0.09
w1118;UAS-CG10417/+;pdfGal4/+1610024.97 ± 0.03
w*;Lar13.2/+149323.54 ± 0.04
Df(2L)TW84,l(2)74i1,amosTftLarTW84/+148623.71 ± 0.11
Df(2L)E55,rdo1hook1LarE55pr1/+168824.32 ± 0.08
w1118;;UAS-Lar/+1410023.57 ± 0.04
w1118;timGal4/+;UAS-Lar/+159324.06 ± 0.07
w1118;;UAS-Lar/pdfGal4167524.17 ± 0.07
w1118;LarDf(2L)E55/Lar13.2;+140AR
w1118;UAS-CanA-14Fmyc/+167524.50 ± 0.20
w1118;UAS-CanA-14Fmyc/timGal4166323.60 ± 0.10
w1118;UAS-CanA-14Fmyc/+;pdfGal4/+1510024.03 ± 0.10
w1118;;UAS-CanA-14Fact-myc/+910023.94 ± 0.11
w1118;timGal4/+;UAS-CanA-14Fact-myc/+150AR
w1118;;UAS-CanA-14Fact-myc/pdfGal4169425.03 ± 0.15
CanA-14F-KO/Y151325.0
CanA-14F-KOiso/Y169423.67 ± 0.08
w1118;GD26216/+1210023.63 ± 0.13
w1118;GD26216/timGal41510024.90 ± 0.13
w1118;GD26216/+;timGal4/+138523.68 ± 0.12
w1118;GD26216/+;pdfGal4/+1510024.30 ± 0.18
  • Adult males were entrained in LD for 3 d and transferred to DD for at least 7 d. Analysis of activity rhythms in DD and fly genotypes are as described in Methods and Materials. n, number of animals tested; % Rhythmic, percentage of rhythmic animals; Period ± SEM, rhythm period in hours ± SEM. Bold “% Rhythmic” values signify < 50% rhythmicity and bold “Period ± SEM” values are significantly different (p ≤ 0.05) from their respective UAS-RNAi/+ control flies. AR, arrhythmic; LD, 1h2:12h light-dark cycle; DD, complete darkness; RNAi, RNA interference.