Table 3 Effect of paromomycin concentration on transformation efficiency
PlasmidaTypeRelative Transformation Efficiency at Different Paromomycin Concentrations (µg/ml)
10203040
pMO449One-promoter (see Figure 1B)100267.13.2
10034113.6
10034115.0
pMO518Two-promoter (see Figure 1A)100978783
1001039590
pMO448Monocistronic (APHVIII-only) control (see Materials and Methods)1001016454
100937671
  • a Data are shown from three (pMO449) or two (pMO518 and pMO448) separate transformation experiments using strain CC-124 and various electroporation methods (see Materials and Methods). In each experiment, the numbers of transformants per micrograms of DNA were normalized to the value obtained at 10 µg/ml paromomycin in that experiment.