Table 1 Activity rhythms of clock cell-specific phosphatase RNAi knockdowns in Drosophila
GenotypeN% RhythmicPeriod Mean ± SEM
w1118;+/GD3018 (puc)1310023.57 ± 0.08
w1118;timGal4/GD30181610024.40 ± 0.05
w1118;GD 3018/+;timGal4/+159324.21 ± 0.16
w1118;GD3018/+;pdfGal4/+1510024.13 ± 0.06
w1118;+/GD3116 (Ptp52F)139223.92 ± 0.15
w1118;timGal4/GD3116169424.50 ± 0.09
w1118;GD3116/+;timGal4/+97824.36 ± 0.22
w1118;GD3116/+;pdfGal4/+87523.92 ± 0.18
w1118;+/GD17123 (TbCMF46)169423.5
w1118;timGal4/GD17123168124.08 ± 0.14
w1118;GD17123/+;timGal4/+1510024.00 ± 0.12
w1118;GD17123/+;pdfGal4/+168823.89 ± 0.13
w1118;+/GD17760 (stg)1610023.56 ± 0.04
w1118;timGal4/GD17760169424.10 ± 0.11
w1118;GD17760/+;timGal4/+1410024.11 ± 0.14
w1118;GD17760/+;pdfGal4/+138523.86 ± 0.15
w1118;+/GD19078 (CG17124)1610023.50 ± 0.06
w1118;timGal4/GD190781610023.81 ± 0.10
w1118;GD19078/+;timGal4/+168824.36 ± 0.09
w1118;GD19078/+;pdfGal4/+1210024.04 ± 0.11
w1118;+/GD21611 (CanB)1610023.03 ± 0.03
w1118;timGal4/GD21611159324.64 ± 0.14
w1118;GD21611/+;timGal4/+1410024.14 ± 0.16
w1118;GD21611/+;pdfGal4/+1610023.66 ± 0.09
w1118;+/GD25317 (Pp4-19C)1410023.71 ± 0.08
w1118;timGal4/GD253171410024.93 ± 0.09
w1118;GD25317/+;timGal4/+159324.39 ± 0.15
w1118;GD25317/+;pdfGal4/+1610023.72 ± 0.09
w1118;+/GD27232 (Ptp4E)1610023.50 ± 0.08
w1118;timGal4/GD272321610024.41 ± 0.05
w1118;GD27232/+;timGal4/+87524.50 ± 0.20
w1118;GD27232/+;pdfGal4/+1310024.54 ± 0.88
w1118;+/GD32283 (CanA1)1610023.50 ± 0.04
w1118;timGal4/GD32283149324.39 ± 0.23
w1118;GD32283/+;timGal4/+169424.53 ± 0.10
w1118;GD32283/+;pdfGal4/+149324.00 ± 0.13
w1118;+/GD32956 (CG17598)169423.90 ± 0.10
w1118;timGal4/GD32956159324.54 ± 0.09
w1118;GD32956/+;timGal4/+144324.58 ± 0.08
w1118;GD32956/+;pdfGal4/+169424.20 ± 0.08
w1118;+/GD35025 (Pp1-87B)1510023.83 ± 0.10
w1118;timGal4/GD350251610024.63 ± 0.08
w1118;GD35025/+;timGal4/+1310024.23 ± 0.12
w1118;GD35025/+;pdfGal4/+1610024.34 ± 0.12
w1118;+/GD41912 (Ptpa)1610023.69 ± 0.09
w1118;timGal4/GD419121510024.57 ± 0.04
w1118;GD41912/+;timGal4/+158024.04 ± 0.15
w1118;GD41912/+;pdfGal4/+1110024.23 ± 0.16
w1118;+/GD41924 (mts)1610023.53 ± 0.03
w1118;timGal4/GD41924168825.14 ± 0.15
w1118;GD41924/+;timGal4/+1110024.73 ± 0.08
w1118;GD41924/+;pdfGal4/+149324.73 ± 0.08
w1118;+/GD42051 (sds22)148623.71 ± 0.09
w1118;timGal4/GD420511610024.81 ± 0.06
w1118;GD42051/+;timGal4/+169424.87 ± 0.11
w1118;GD42051/+;pdfGal4/+1410024.14 ± 0.12
w1118;+/GD45415 (Mkp3)159323.78 ± 0.09
w1118;timGal4/GD454151510024.67 ± 0.08
w1118;GD45415/+;timGal4/+158024.33 ± 0.15
w1118;GD45415/+;pdfGal4/+149324.38 ± 0.10
w1118;+/GD49671 (Pp2a-29B)1510023.70 ± 0.06
w1118;timGal4/GD4967130
w1118;GD49671/+;timGal4/+1510026.13 ± 0.09
w1118;GD49671/+;pdfGal4/+169426.17 ± 0.20
w1118;+/KK100121 (CG6380)1610023.63 ± 0.07
w1118;timGal4/KK100121142923.38 ± 0.13
w1118;KK100121/+;timGal4/+90AR
w1118;KK100121/+;pdfGal4/+15723.5
w1118;+/KK100178 (CG17746)168823.43 ± 0.05
w1118;timGal4/KK100178120AR
w1118;KK100178/+;timGal4/+123325.88 ± 0.32
w1118;KK100178/+;pdfGal4/+120AR
w1118;+/KK100216 (CG2104)169423.5
w1118;timGal4/KK100216166323.55 ± 0.05
w1118;KK100216/+;timGal4/+155323.75 ± 0.15
w1118;KK100216/+;pdfGal4/+1410023.61 ± 0.06
w1118;+/KK100283 (Mapmodulin)1510023.07 ± 0.06
w1118;timGal4/KK100283168123.89 ± 0.21
w1118;KK100283/+;timGal4/+149324.04 ± 0.18
w1118;KK100283/+;pdfGal4/+1610023.78 ± 0.11
w1118;+/KK100593 (Gbs-70E)1610023.53 ± 0.03
w1118;timGal4/KK100593135425.21 ± 0.33
w1118;KK100593/+;timGal4/+168825.46 ± 0.17
w1118;KK100593/+;pdfGal4/+13823.5
w1118;+/KK100914 (CG42327)1610023.53 ± 0.03
w1118;timGal4/KK1009141610023.78 ± 0.11
w1118;KK100914/+;timGal4/+158023.5
w1118;KK 100914/+;pdfGal4/+1610023.53 ± 0.03
w1118;+/KK101257 (Ppm1)288923.50 ± 0.06
w1118;timGal4/KK101257152024.67 ± 0.44
w1118;KK101257/+;timGal4/+132324.67 ± 0.17
w1118;KK101257/+;pdfGal4/+154023.42 ± 0.08
w1118;+/KK101335 (CG10376)1610023.5
w1118;timGal4/KK101335169424.30 ± 0.12
w1118;KK101335/+;timGal4/+168824.32 ± 0.53
w1118;KK101335/+;pdfGal4/+168823.75 ± 0.17
w1118;+/KK101406 (wdb)158723.42 ± 0.05
w1118;timGal4/KK101406168824.20 ± 0.09
w1118;KK101406/+;timGal4/+155325.86 ± 0.23
w1118;KK101406/+;pdfGal4/+139223.83 ± 0.09
w1118;+/KK101474 (Clb2)1610023.69 ± 0.09
w1118;timGal4/KK101474168823.43 ± 0.16
w1118;KK101474/+;timGal4/+168823.61 ± 0.14
w1118;KK101474/+;pdfGal4/+1410023.57 ± 0.05
w1118;+/KK101547 (I-2)169423.47 ± 0.03
w1118;timGal4/KK101547144325.25 ± 0.35
w1118;KK101547/+;timGal4/+163125.40 ± 0.17
w1118;KK101547/+;pdfGal4/+132324.83 ± 0.59
w1118;+/KK101997 (PpV)1610023.44 ± 0.04
w1118;timGal4/KK101997125023.50 ± 0.20
w1118;KK101997/+;timGal4/+126724.81 ± 0.34
w1118;KK101997/+;pdfGal4/+100AR
w1118;+/KK102021 (PpY-55A)1610023.47 ± 0.03
w1118;timGal4/KK102021159324.21 ± 0.17
w1118;KK102021/+;timGal4/+146423.78 ± 0.18
w1118;KK102021/+;pdfGal4/+1410023.89 ± 0.18
w1118;+/KK102060 (PpN-58A)289623.50 ± 0.03
w1118;timGal4/KK102060156024.06 ± 0.18
w1118;KK102060/+;timGal4/+156024.39 ± 0.14
w1118;KK102060/+;pdfGal4/+139223.92 ± 0.18
w1118;+/KK102071 (CG14297)2510023.53 ± 0.05
w1118;timGal4/KK102071156723.55 ± 0.17
w1118;KK102071/+;timGal4/+167524.00 ± 0.19
w1118;KK102071/+;pdfGal4/+169423.50 ± 0.05
w1118;+/KK102397 (Ptp36E)1610023.63 ± 0.07
w1118;timGal4/KK1023971410024.21 ± 0.11
w1118;KK102397/+;timGal4/+159324.12 ± 0.14
w1118;KK102397/+;pdfGal4/+158023.54 ± 0.09
w1118;+/KK102474 (CG31469)1510023.73 ± 0.08
w1118;timGal4/KK1024741610024.59 ± 0.12
w1118;KK102474/+;timGal4/+149323.73 ± 0.09
w1118;KK102474/+;pdfGal4/+169423.77 ± 0.08
w1118;+/KK103044 (Gbs-76A)168123.38 ± 0.06
w1118;timGal4/KK103044167523.71 ± 0.11
w1118;KK103044/+;timGal4/+156724.85 ± 0.10
w1118;KK103044/+;pdfGal4/+1410023.93 ± 0.07
w1118;+/KK103144 (Pp2B-14D)169423.47 ± 0.06
w1118;timGal4/KK103144165624.11 ± 0.27
w1118;KK103144/+;timGal4/+107024.21 ± 0.32
w1118;KK103144/+;pdfGal4/+139223.88 ± 0.13
w1118;+/KK103317 (CG32568)1610023.53 ± 0.03
w1118;timGal4/KK103317169423.83 ± 0.12
w1118;KK103317/+;timGal4/+1210023.96 ± 0.12
w1118;KK103317/+;pdfGal4/+1610023.53 ± 0.05
w1118;+/KK103354 (CG7115)1510023.6 ± 0.05
w1118;timGal4/KK103354148624.88 ± 0.2
w1118;KK103354/+;timGal4/+75425.0 ± 0.18
w1118;KK103354/+;pdfGal4/+120AR
w1118;+/KK103357 (Cep97)1510023.57 ± 0.08
w1118;timGal4/KK103357122523.83 ± 0.27
w1118;KK103357/+;timGal4/+13825.0
w1118;KK103357/+;pdfGal4/+100AR
w1118;+/KK103627 (cdc14)1610023.34 ± 0.06
w1118;timGal4/KK103627157324.05 ± 0.22
w1118;KK103627/+;timGal4/+159325.32 ± 0.14
w1118;KK103627/+;pdfGal4/+13826.5
w1118;+/KK103740 (Ptpmeg)169423.40 ± 0.05
w1118;timGal4/KK103740168823.54 ± 0.03
w1118;KK103740/+;timGal4/+158723.77 ± 0.14
w1118;KK103740/+;pdfGal4/+1610023.5
w1118;+/KK104081 (CG32812)1110023.5
w1118;timGal4/KK1040811610023.63 ± 0.07
w1118;KK104081/+;timGal4/+168823.61 ± 0.08
w1118;KK104081/+;pdfGal4/+169423.73 ± 0.09
w1118;+/KK104167 (tws)135423.14 ± 0.09
w1118;timGal4/KK104167158723.85 ± 0.14
w1118;KK104167/+;timGal4/+127523.89 ± 0.20
w1118;KK104167/+;pdfGal4/+169423.80 ± 0.13
w1118;+/KK104211 (PpD6)159323.42 ± 0.04
w1118;timGal4/KK104211142924.88 ± 0.52
w1118;KK104211/+;timGal4/+125825.14 ± 0.37
w1118;KK104211/+;pdfGal4/+12825.5
w1118;+/KK104374 (Mkp)148623.5
w1118;timGal4/KK104374168823.46 ± 0.04
w1118;KK104374/+;timGal4/+149324.00 ± 0.17
w1118;KK104374/+;pdfGal4/+1510024.03 ± 0.06
w1118;+/KK104427 (Ptpmeg2)169423.57 ± 0.12
w1118;timGal4/KK104427130AR
w1118;KK104427/+;timGal4/+135425.00 ± 0.68
w1118;KK104427/+;pdfGal4/+101023.5
w1118;+/KK104452 (PpD5)1610023.50 ± 0.04
w1118;timGal4/KK104452164425.29 ± 0.33
w1118;KK104452/+;timGal4/+156724.35 ± 0.21
w1118;KK104452/+;pdfGal4/+131523.25 ± 0.18
w1118;+/KK104677 (flw)1510023.5
w1118;timGal4/KK104677169424.5
w1118;KK104677/+;timGal4/+1610023.97 ± 0.13
w1118;KK104677/+;pdfGal4/+1610024.31 ± 0.08
w1118;+/KK104729 (CG11597)168823.29 ± 0.07
w1118;timGal4/KK104729136923.61 ± 0.07
w1118;KK104729/+;timGal4/+119123.70 ± 0.08
w1118;KK104729/+;pdfGal4/+169423.80 ± 0.12
w1118;+/KK104761 (Ptp69D)1510023.53 ± 0.03
w1118;timGal4/KK104761121724.75 ± 0.18
w1118;KK104761/+;timGal4/+135425.43 ± 0.34
w1118;KK104761/+;pdfGal4/+80AR
w1118;+/KK104774 (PTPMT1)1510023.47 ± 0.03
w1118;timGal4/KK104774159324.18 ± 0.14
w1118;KK104774/+;timGal4/+148624.63 ± 0.24
w1118;KK104774/+;pdfGal4/+88823.36 ± 0.09
w1118;+/KK104785 (Dd)1610023.03 ± 0.03
w1118;timGal4/KK1047851610023.94 ± 0.13
w1118;KK104785/+;timGal4/+1610023.97 ± 0.11
w1118;KK104785/+;pdfGal4/+169423.70 ± 0.15
w1118;+/KK104860 (mop)1610023.5
w1118;timGal4/KK104860139224.25 ± 0.14
w1118;KK104860/+;timGal4/+158024.83 ± 0.31
w1118;KK104860/+;pdfGal4/+168123.62 ± 0.11
w1118;+/KK104884 (MKP-4)169023.37 ± 0.04
w1118;timGal4/KK104884153324.40 ± 0.40
w1118;KK104884/+;timGal4/+126725.44 ± 0.18
w1118;KK104884/+;pdfGal4/+112724.00 ± 0.41
w1118;+/KK105122 (CG13197)168823.46 ± 0.03
w1118;timGal4/KK105122169423.83 ± 0.12
w1118;KK105122/+;timGal4/+1610023.69 ± 0.12
w1118;KK105122/+;pdfGal4/+1510024.07 ± 0.16
w1118;+/KK105249 (Pp2C)1610023.33 ± 0.04
w1118;timGal4/KK105249163124.20 ± 0.30
w1118;KK105249/+;timGal4/+136225.56 ± 0.20
w1118;KK105249/+;pdfGal4/+1610023.78 ± 0.08
w1118;+/KK105399 (PPP4R2r)1310023.65 ± 0.05
w1118;timGal4/KK105399122523.17 ± 0.17
w1118;KK105399/+;timGal4/+70AR
w1118;KK105399/+;pdfGal4/+97823.00 ± 0.25
w1118;+/KK105483 (alph)1610023.5
w1118;timGal4/KK105483168823.89 ± 0.10
w1118;KK105483/+;timGal4/+169423.73 ± 0.13
w1118;KK105483/+;pdfGal4/+1310024.50 ± 0.13
w1118;+/KK105484 (CG15528)137723.25 ± 0.08
w1118;timGal4/KK105484165023.69 ± 0.26
w1118;KK105484/+;timGal4/+142923.63 ± 0.27
w1118;KK105484/+;pdfGal4/+1510023.90 ± 0.05
w1118;+/KK105525 (Pp1α-96A)1610023.57 ± 0.05
w1118;timGal4/KK105525105025.20 ± 0.34
w1118;KK105525/+;timGal4/+138525.50 ± 0.13
w1118;KK105525/+;pdfGal4/+169424.73 ± 0.12
w1118;+/KK105565 (I-t)1410023.5
w1118;timGal4/KK105565155323.63 ± 0.08
w1118;KK105565/+;timGal4/+165623.56 ± 0.05
w1118;KK105565/+;pdfGal4/+115523.47 ± 0.06
w1118;+/KK105568 (CG6036)1610023.5
w1118;timGal4/KK105568165624.28 ± 0.12
w1118;KK105568/+;timGal4/+147123.90 ± 0.19
w1118;KK105568/+;pdfGal4/+129223.68 ± 0.10
w1118;+/KK105674 (CG5026)1610023.47 ± 0.03
w1118;timGal4/KK1056741610024.19 ± 0.12
w1118;KK105674/+;timGal4/+1610023.97 ± 0.13
w1118;KK105674/+;pdfGal4/+1610023.5
w1118;+/KK106098 (CG7378)1610023.53 ± 0.03
w1118;timGal4/KK106098166924.14 ± 0.27
w1118;KK106098/+;timGal4/+169425.07 ± 0.15
w1118;KK106098/+;pdfGal4/+135424.00 ± 0.49
w1118;+/KK106180 (CG10417)169423.50 ± 0.05
w1118;timGal4/KK106180141424.75 ± 0.18
w1118;KK106180/+;timGal4/+157324.64 ± 0.27
w1118;KK106180/+;pdfGal4/+161323.25 ± 0.18
w1118;+/KK106253 (Fcp1)1610023.53 ± 0.03
w1118;timGal4/KK1062531110024.36 ± 0.15
w1118;KK106253/+;timGal4/+410023.75 ± 0.22
w1118;KK106253/+;pdfGal4/+1610023.47 ± 0.03
w1118;+/KK107057 (PP2A-B’)318723.48 ± 0.06
w1118;timGal4/KK107057165022.25 ± 0.34
w1118;KK107057/+;timGal4/+124222.30 ± 0.34
w1118;KK107057/+;pdfGal4/+168123.65 ± 0.14
w1118;+/KK107386 (PpD3)810023.5
w1118;timGal4/KK1073861210024.04 ± 0.12
w1118;KK107386/+;timGal4/+1110023.68 ± 0.16
w1118;KK107386/+;pdfGal4/+1510023.57 ± 0.04
w1118;+/KK107621 (CG4733)1610023.56 ± 0.04
w1118;timGal4/KK107621168123.65 ± 0.09
w1118;KK107621/+;timGal4/+1610023.66 ± 0.07
w1118;KK107621/+;pdfGal4/+1510023.63 ± 0.10
w1118;+/KK107770 (Pp1-13C)1510023.43 ± 0.04
w1118;timGal4/KK1077701610023.44 ± 0.08
w1118;KK107770/+;timGal4/+169423.63 ± 0.07
w1118;KK107770/+;pdfGal4/+159323.43 ± 0.05
w1118;+/KK107996 (Lar)1610023.53 ± 0.03
w1118;timGal4/KK107996122524.17 ± 0.27
w1118;KK107996/+;timGal4/+160AR
w1118;KK107996/+;pdfGal4/+130AR
w1118;+/KK107998 (ssh)1610023.5
w1118;timGal4/KK107998159324.07 ± 0.10
w1118;KK107998/+;timGal4/+1510024.30 ± 0.15
w1118;KK107998/+;pdfGal4/+1610023.44 ± 0.04
w1118;+/KK108071 (eya)1610023.5
w1118;timGal4/KK108071168824.11 ± 0.13
w1118;KK108071/+;timGal4/+1610023.66 ± 0.09
w1118;KK108071/+;pdfGal4/+1610023.5
w1118;+/KK108352 (csw)1510023.60 ± 0.05
w1118;timGal4/KK1083521610024.41 ± 0.08
w1118;KK108352/+;timGal4/+169424.23 ± 0.09
w1118;KK108352/+;pdfGal4/+158723.62 ± 0.08
w1118;+/KK108505 (Ptp99A)1610023.5
w1118;timGal4/KK108505139223.71 ± 0.11
w1118;KK108505/+;timGal4/+1610023.56 ± 0.04
w1118;KK108505/+;pdfGal4/+1610023.75 ± 0.09
w1118;+/KK108744 (CG10089)158723.38 ± 0.10
w1118;timGal4/KK108744148624.00 ± 0.12
w1118;KK108744/+;timGal4/+154724.29 ± 0.14
w1118;KK108744/+;pdfGal4/+168123.73 ± 0.14
w1118;+/KK108802 (CG8509)1410023.54 ± 0.03
w1118;timGal4/KK108802149324.00 ± 0.09
w1118;KK108802/+;timGal4/+168124.15 ± 0.14
w1118;KK108802/+;pdfGal4/+159323.79 ± 0.07
w1118;+/KK108859 (NIPP1)158023.46 ± 0.09
w1118;timGal4/KK108859167523.86 ± 0.13
w1118;KK108859/+;timGal4/+128324.71 ± 0.23
w1118;KK108859/+;pdfGal4/+1510023.80 ± 0.09
w1118;+/KK108888 (Ptp61F)1610023.72 ± 0.08
w1118;timGal4/KK108888169423.70 ± 0.14
w1118;KK108888/+;timGal4/+169423.67 ± 0.09
w1118;KK108888/+;pdfGal4/+169423.70 ± 0.10
w1118;+/KK109147 (PP1-Y2)1610023.75 ± 0.06
w1118;timGal4/KK109147141423.5
w1118;KK109147/+;timGal4/+111824.75 ± 0.18
w1118;KK109147/+;pdfGal4/+126725.06 ± 0.78
w1118;+/KK109622 (CG14411)169423.53 ± 0.03
w1118;timGal4/KK1096221610024.50 ± 0.13
w1118;KK109622/+;timGal4/+127524.06 ± 0.17
w1118;KK109622/+;pdfGal4/+169423.60 ± 0.10
w1118;+/KK109858 (CanA-14F)1610023.5
w1118;timGal4/KK109858114524.08 ± 0.27
w1118;KK109858/+;timGal4/+101023.5
w1118;KK109858/+;pdfGal4/+110AR
w1118;+/KK110167 (CG3632)1610023.5
w1118;timGal4/KK110167169423.70 ± 0.10
w1118;KK110167/+;timGal4/+158723.73 ± 0.21
w1118;KK110167/+;pdfGal4/+138523.45 ± 0.04
w1118;+/KK110360 (Phlpp)168823.40 ± 0.07
w1118;timGal4/KK110360149324.00 ± 0.08
w1118;KK110360/+;timGal4/+1610023.75 ± 0.10
w1118;KK110360/+;pdfGal4/+168123.81 ± 0.27
w1118;+/KK110443 (Ptp10D)1610023.59 ± 0.05
w1118;timGal4/KK110443157324.05 ± 0.25
w1118;KK110443/+;timGal4/+136925.28 ± 0.26
w1118;KK110443/+;pdfGal4/+147123.55 ± 0.22
w1118;+/KK110595 (IA-2)1610023.72 ± 0.12
w1118;timGal4/KK110595126724.42 ± 0.08
w1118;KK110595/+;timGal4/+166324.70 ± 0.08
w1118;KK110595/+;pdfGal4/+58024.50 ± 0.31
w1118;+/KK110786 (CG3530)1610023.5
w1118;timGal4/KK110786168825.36 ± 0.08
w1118;KK110786/+;timGal4/+169425.37 ± 0.11
w1118;KK110786/+;pdfGal4/+14727.0
  • Adult males were entrained in LD for 3 d and transferred to DD for at least 7 d. Analysis of activity rhythms in DD and fly genotypes are as described in Methods and Materials. For each RNAi line tested, the gene name or CG number is listed in parenthesis after the control RNAi only genotype. N, number of animals tested; % Rhythmic, percentage of rhythmic animals; Period ± SEM, rhythm period in hours ± SEM. Genotypes that lack SEM values all fell into the same half hour period increment. Bold “% Rhythmic” values signify <50% rhythmicity, bold “Period ± SEM” values are significantly different (p ≤ 0.05) from their respective UAS-RNAi/+ control flies, and “—” indicates that flies of the given genotype did not survive the run of the assay. AR, arrhythmic; LD, 12h:12h light-dark cycle; DD, complete darkness; RNAi, RNA interference.