Table 1 Sequencing statistics
Mean Target Coverage% of Target Bases ≥10×Total Variants on 3RProtein Coding Variants on 3R
No FilterRD ≥ 15, GQ ≥ 30No FilterRD ≥ 15, GQ ≥ 30
One Lane Sequences22.7 (±6.2)94.90 (±4.37)310.2 (±68.2)83.7 (±25.3)23.8 (±6.1)12.5 (±4.8)
Two Lane Sequences49.7 (±8.2)96.50 (±0.08)229.4 (±39.7)139.6 (±25.4)21.8 (±0.4)16.4 (±0.9)
P Value (t Test)nsP < 0.05P < 0.001nsP < 0.05
  • Mutation predictions for 16 mutants strains sequenced in either one or two Illumina lanes, as indicated. Results are reported prior to selective validation by Sanger sequencing, and include false positives. RD (read depth) and GQ (genome quality) represent filters for sequence quality. See Results section on identification of mutations for details. Errors represent ± 1 SD.