Table 1 Testing viability of chromosomal pairing factor knockdown in vivo
Adult Viability with shRNA Expression
GeneBDSC shRNA Stock NumberCha-GAL4 > shRNAα-tubulin-GAL4 > shRNA
CG246933,736N/a
chb34,669N/a
Dhc64C36,698N/a
Klp61f33,685N/a
Nlp33,688N/a
shtd38,531N/a
SkpA32,991N/a
slmb33,986N/a
Tlk33,983N/a
Arp132,032+
aurB28,691+
cal141,716+
CG723627,505+
CKIα25,786+
Cul129,520+
Det51,837+
E(bx)33,658+
fzy40,933+
Hsc70Cb33,742++
ida34,552+
Lis-135,043+
Nc73EF33,686+
pbl28,343+
polo33,042+
su(Hw)34,006++
w33,623++
  • shRNAs from the TRiP library against the listed genes, which have been identified in previous studies as playing a role in somatic homolog pairing, were obtained from the BDSC. shRNA expression was driven in cholinergic neurons with the Cha-GAL4 driver, or ubiquitously with the α-tubulin-GAL4 driver. shRNA against the w gene was included as a positive control. shRNA, short hairpin RNA; BDSC, Bloomington Drosophila Stock Center; −, no viable adult flies recovered; N/a, cross not performed; +, viable adult flies recovered; TRiP, Transgenic RNA Interference Project.