Table 2 Sensitivity to MMS of double mutants of genes involved in different aspects of the cell cycle
CategoryMutantFunctionPhenotype with c106aMMS Phenotypeb
Checkpointcds1ΔKinase; S phase/replication checkpointViable>mcm4-c106
Checkpointchk1ΔKinase; G2/damage checkpointSynthetic lethalND
Checkpointmad2ΔSpindle checkpointViable=mcm4-c106
Checkpointrad26ΔCheckpoint proteinSynthetic lethalND
Checkpointrad3ΔKinaseSynthetic lethalND
Cohesionchl1ΔHelicaseViable> mcm4-c106
Cohesionrad21-K1Cohesin proteinViable= rad21-K1
Replicationmcl1-1 tsPart of the FPC/cohesionSickND
Replication-FPCmrc1ΔReplication mutants/FPCSynthetic lethalND
Replication-FPCswi1ΔReplication mutants/FPCSynthetic lethalND
Replication-FPCswi3ΔReplication mutants/FPCSynthetic lethalND
Replicationhsk1-1312DDK kinaseSynthetic lethalND
Replicationdfp1-r35DDK kinaseViable/sickND
Replication, genome stabilityrif1ΔRif1 antagonist of DDKViablerescued
Genome stabilitybrc1ΔGenome stabilityViablemcm4-c106
Replication clamp loaderctf8ΔCohesion-specific clamp loaderViable < temp= mcm4-c106
Replication clamp loaderctf18ΔCohesion-specific clamp loaderViable < temp= mcm4-c106
Genome stabilityfml1ΔHelicase; genome stabilityViable=fml1Δ
Genome stabilitymus81ΔHoliday junction resolvaseSick=mus81
Genome stabilityrqh1ΔHelicase; recombination antagonistViable> mcm4-c106
Genome stabilitysrs2ΔHelicase; recombination regulatorViable>mcm4-c106
Repaireso1etaEso1- Polη fusion; deletes polymerase domain. Error prone repairViablemcm4c106
Repairexo1ΔExonuclease IViable= exo1Δ
Repairmms2ΔUbiquitin ligase; error free repairViable> mms2Δ
Repairpcn1-K164RPCNA; ubiquitin site mutantViable= pcn1-K164R
RepairpolkΔPolκ; error prone repairViable>mcm4-c106
Repairrad51ΔHomologous recombinationViable=rad51Δ
Repairrad8ΔUbiquitin ligase-helicase; error free repairViable>rad8Δ
Repairrev1ΔDeoxycytidyl transferase; error prone repairViablemcm4-c106
Repairrev3ΔPolζ Error prone repairViable<mcm4-c106
Repairrhp18ΔPCNA ubiquitin ligaseViable= rhp18Δ
Repairubc13ΔUbiquitin ligase; error freeViable> ubc13Δ
chp1ΔHeterochromatin proteinViable=mcm4-c106
cig2Δ/_cyc17S phase cyclinViablemcm4-c106
reb1ΔTranscription terminationViable < temp=mcm4-c106
swi6ΔHeterochromatin proteinViable= mcm4-c106
rad22ΔViable/slow growingND
cdc20- M10(polε)ViableND
cdc6- 23(polδ)Synthetic lethalNA
psf2tsComponent of the GINS replication complexViable>mcm4-c106
pol1-1(polα)ViableND
rad50ΔSynthetic lethalNA
  • Double mutant phenotypes between mcm4-c106 and other mutants in the indicated classes. MMS, methyl methanesulfonate; ND, Not Determined; FPC, fork protection complex; DDK, Dbf4-dependent kinase; PCNA, proliferating cell nuclear antigen; NA, Not Applicable.

  • a Phenotype: viable means no change in temperature sensitivity. Synthetic lethal is dead at temperatures. Sick shows reduced growth rate at all temperatures. <temp has reduced maximum growth temperature.

  • b MMS phenotype is determined relative to the most sensitive parent (indicated) being > (greater sensitivity), < (less sensitive), or = (equal sensitivity).