Table 2 Pairwise t-tests show which transgenes inserted at which insertion sites alter pigmentation relative to D. melanogaster tan mutants, as well as which landing sites show evidence of functional differences between the D. americana and D. novamexicana tan alleles
51C51C58A58A86Fa86Fa86Fb86Fb102D
tan mutantD. amerD. novaD. amerD. novaD. amerD. novaD. amerD. novaD. amer
51CD. amer4 × 10−9
51CD. nova8 × 10−80.411
58AD. amer5 × 10−183 × 10−102 × 10−11
58AD. nova4 × 10−121 × 10−31 × 10−45 × 10−5
86FaD. amer4 × 10−243 × 10−141 × 10−150.3172 × 10−7
86FaD. nova2 × 10−214 × 10−71 × 10−81 × 10−30.0932 × 10−6
86FbD. amer3 × 10−231 × 10−159 × 10−170.0122 × 10−90.0641 × 10−8
86FbD. nova9 × 10−171 × 10−77 × 10−90.0640.0112 × 10−30.1952 × 10−5
102DD. amer0.1251 × 10−41 × 10−39 × 10−143 × 10−84 × 10−158 × 10−119 × 10−171 × 10−11
102DD. nova0.0883 × 10−77 × 10−67 × 10−178 × 10−112 × 10−233 × 10−203 × 10−222 × 10−150.706
  • p-values adjusted by the Benjamini and Hochberg method from all possible pairwise t-tests using unpooled standard deviation are shown for each pair of genotypes compared. The tan mutant column shows results from comparisons between each transgenic genotype and the tan mutant (no transgene) control. Note that neither transgene darkened pigmentation relative to the tan mutant when inserted at landing site 102D. Highlighted boxes indicate comparisons between the D. americana and D. novamexicana tan alleles inserted at the same landing site. Significant differences (p < 0.05) in median pigmentation were observed for transgenes inserted at 58A, 86Fa, and 86Fb, but not 51C or 102D. D. amer, D. Americana; D. nova, D. novamexicana.