Table 1 Chimera generation and germline transmission from targeted embryonic stem cell clones
GeneAlleleCloneSourceParental ES Cell LineEmbryos InjectedChimeras Generated Germline Transmission (# Screened)
Cav3CAV3tm1(Komp)Vlcg11604B-F10KOMPVGB632m, 29b76
Il17rdIl17rdtm1a(EUCOMM)HmguHEPD0636_8_G08EUCOMMJM8A3.N140m, 14b2M,3F2
Mir199bMir199btm1(mirKO)WtsimirKO_ES_PuDtk_11D9Sanger miR knockoutsJM8A369m, 61b4Ma0 (>49)a
mirKO_ES_PuDtk_11D12Sanger miR knockoutsJM8A366m
Lgr4Lgr4tm1a(EUCOMM)HmguHEPD0729_3_A08EUCOMMJM8A3.N140m, 32b4M0 (no pups born)
Col1a1Commercially developedApplied StemCellJM8A3.N173m, 25b3M1
Dock7Dock7tm1a(EUCOMM)WtsiEPD0821_4_G02EUCOMMJM8A3.N176m, 56b6M0 correctly  targetedb
  • The source of targeted ES cell lines for the genes shown is indicated. Either morula (m) or blastocysts (b) were injected, and resultant chimeras were bred to monitor germline transmission. For those lines without germline transmission, the total number of pups screened is shown in parentheses. m, morula; b, blastocyst; M, male; F, female.

  • a Both clones were injected, resulting in only one chimera.

  • b Please see subsequent results for details on the Dock7 tm1a allele.