Table 4 Germline transmission rate of loxP sites in the Dock7 cKO1 model
N0 GenerationN1 Generation
GenotypeGenotypeLoxP Location
InjectionMouse ID#LoxP4LoxP5Percentage of Pups with GenotypeLoxP4LoxP5(cis/trans)
cKO1-13L,+L,+16/47 (34%)mL*,+Lm,+cis
cKO1-2114+L,+1/4 (25%)+L,+
116LL18/37 (49%)mLm,+Lm,+cis
117L,++3/27 (11%)*L*,++
120+L2/5 (40%)*+L*,+
122LL9/22 (41%)*L*,+aL*,+acisa
2/22 (9%)+L*,+
1/22 (5%)L*,++
139Ln13/27 (48%)*L*,++
142L,+L0/28 (0%)++not tested
143+L14/36 (39%)*+L*,+
  • N0 mice from the Dock7 cKO1 model with one or two loxP sites were bred and the N1 generation was assessed for the floxed allele and germline transmission of the loxP sites. Genotype is indicated by (L) loxP site, (+) wild type, and (n) to indicate no genotyping band and likely a null allele. (*) Represents mice with loxP sites containing correct sequence and (m) represent mice with a mutated loxP sequence. Amplification of DNA from tail/toe clips was performed using primer pairs loxP4A F/R or loxP4B F/R for loxP4 which provided identical results and could be used interchangeably. Amplification of DNA from tail/toe clips was formed using primer pairs loxP5A F/R or loxP5B F/R for loxP5 which provided identical results and could be used interchangeably.

  • a Mice containing the correctly targeted floxed allele.