Table 1 Sex-differential expression in Canton S and Berlin strains
ExonsGenes
WT Female to Male ComparisonWT Female to Male Comparison
Male-biasedFemale-BiasedMale-BiasedFemale-Biased
Canton S2268568617431672
Berlin107023657831611
Both231376205246
WT Female to dsxD ComparisonaWT Female to dsxD Comparisona
Male-BiasedFemale-BiasedMale-BiasedFemale-Biased
Canton S340901303445
Berlin7820769168
Both1229520
WT to dsx Null ComparisonsbWT to dsx Null Comparisonsb
Male-BiasedFemale-BiasedMale-BiasedFemale-Biased
Canton S275706239358
Berlin5212044105
Both1118413
  • Each contrast compared the natural log of the RPKM normalized expression in wild-type females and males, wild-type females and dsxD pseudo males, and in wild-type females and dsx null females or wild-type males and dsx null males. A cut-off of FDR < 0.05 was used.

  • a For each exonic region, the wild-type comparison was considered first, and if sex-biased in the wild-type comparison, the exon was considered sex-differentially expressed and regulated by dsx if there was also significant sex-differential expression that was biased in the same direction in the wild-type female to dsxD comparison.

  • b If the exon was identified as a putative dsx target as in (a), then it was tested for a significant difference in either or both dsx null comparisons. Both exon level and corresponding gene level counts are reported.