Table 3 Genes selected for real-time PCR validation and the fold change in the postlarval stage (Y5) compared with the four-spiral whorls stage (competent larvae J4) for both RNA-seq and RT-PCR analysis
log2.fold_change (Y5 vs. J4)
Gene IDGene NameExpRNA-SeqRT-PCRSame Trend
c95470_g1vdg3−9.55**−6.00**Y
c131243_g1CTSV−8.40**−8.90*Y
c139260_g1CAV1−7.39**−1.84**Y
c129336_g1Fcer2−6.30**−7.46**Y
c125484_g1SARP-19−5.75**−2.01**Y
c128565_g1CA2−5.63**−4.23*Y
c145993_g2ACT1−5.02**−1.91*Y
c127784_g1Lrp1−4.71**−4.06**Y
c145412_g1nas-14−4.59**−4.35*Y
c119967_g1CRISPLD2−4.29**−2.67*Y
c150152_g1ENTPD34.56**8.20*Y
c113545_g2WBSCR274.50**5.73**Y
c94349_g1pxdn4.49**5.50*Y
c156029_g2xynA4.43**7.30**Y
c117375_g2TNF4.38**7.79**Y
c147483_g1Lrp44.34**6.44*Y
c149073_g1Arsj4.31**8.56**Y
c143535_g1LCP13.89**4.33**Y
c135964_g1IRX123.85**6.37**Y
c118662_g1Lrp23.81**4.34**Y
  • Genes with the same trend (up or downregulation) as shown by both RNA-seq and RT-PCR analysis are indicated by “Y” (yes). * P < 0.05, ** P < 0.01 (significant difference between J4 and Y5 stages). Exp, expression trend; “↑”upregulated expression; “↓” downregulated expression.