Table 1 Currently available plasmids for the genomic tagging procedure
Blasticidin ResistancePuromycin ResistanceInducible Blasticidin Resistance
TypeTagNameAddgene NumberNameAddgene NumberNameAddgene Number
C-terminal tag
eGFPpMH352528pSK2372851
twin-StreppIW152530pSK2472852
2 × FlagpMH452529pSK2572853
TEV-V5(long)pSK3272854
TEV-V5(short)pKF29674773pKF29774774
PhiC31 attP sitepSK1572885pSK4174886
Targeting vectorAct5C-eGFPpIS174887
N-terminal tag
Base vectorpRB3672861pRB3572866pKF29272856
3 × FlagpRB3472862pRB3372867pKF29372857
1 × StreppRB3272863pRB3172868pKF29472858
Strep-His8-Strep-TEVpRB3872864pRB3772869pKF29072859
eGFPpRB4072865pRB3972870pRB3072860
sgRNA expressionsnRNA:U6:96Ac prom.pRB1752527
FLP-recombinationNLS-FLP (P2->S)pKF29572871
  • sgRNA, single guide RNA.