Table 3 The empirical genomic coverages (EgC) for three restriction enzyme combinations (PM = PstI + MspI, AB = AvaII + BfaI, and HH = HinfI + HpyCH4IV) and the ratio of the EgC relative to PM (Ratio to PM) in six dicot and six monocot species
PMABHH
Plant SpeciesGSaContig × LengthaEgC (%)Contig × LengthEgC (%)Ratio to PMContig × LengthEgC (%)Ratio to PM
Dicot
 Arabidopsis thaliana15617,352 × 2080.7451,231 × 2182.283.09111,175 × 1964.456.03
 Pisum sativum476827,703 × 1750.10355,620 × 1270.959.35203,041 × 1300.555.45
 Linum grandiflorum68553,747 × 1731.36122,437 × 1422.551.87202,992 × 1474.373.21
 Carthamus tinctorius136422,686 × 1940.32166,661 × 1501.835.67159,252 × 1481.725.34
 Glycine max110045,759 × 1710.71222,148 × 1653.334.67224,215 × 1633.334.67
 Sinapis alba48939,423 × 1911.54141,589 × 1674.833.13182,384 × 1726.414.16
 Mean0.802.634.633.474.81
Monocot
 Aegilops umbellulata4939128,544 × 1640.43294,115 × 1260.751.76311,323 × 1480.932.19
 Pseudoroegneria spicata4450186,910 × 1590.67466,001 × 1291.352.02365,274 × 1511.241.85
 Agropyron cristatum6969264,949 × 1470.56549,794 × 1261.001.78439,060 × 1470.921.65
 Zea mays266584,632 × 1820.58295,130 × 1361.512.62293,728 × 1471.622.81
 Elymus lanceolatus8240275,761 × 1570.52368,636 × 1310.591.12416,130 × 1450.731.39
 Oryza sativa48975,742 × 1953.02164,171 × 1906.362.11209,403 × 1847.902.61
 Mean0.961.931.902.222.08
Overall mean0.882.283.272.853.45
  • a GS, genome size in Mb obtained from Royal Botanic Gardens, Kew Plant DNA C-values Database; GS is not available for L. grandiflorum, so GS for the related flax species (L. usitatissiumum) was used. Contig × length = number of contigs × average length (bp) per contig.