Table 3 Ectopic expression of WOR1 can overcome the effects of the wor4 deletion
White-to-Opaque Switching
StrainMedia ConditionSwitching Frequency (%)nNotesa
Wild Type, pMET3-BlankRepressing<0.601681
Wild Type, pMET3-BlankInducing<0.323081
Wild Type, pMET3-WOR1Repressing<0.472142
Wild Type, pMET3-WOR1Inducing98.963842
wor4/wor4, pMET3-BlankRepressing<0.82122
wor4/wor4, pMET3-BlankInducing<0.31327
wor4/wor4, pMET3-WOR1Repressing<0.74136
wor4/wor4, pMET3-WOR1Inducing100.00315
wor4/wor4, pMET3-WOR1Repressing<0.49203
wor4/wor4, pMET3-WOR1Inducing99.54434
Opaque-to-White Switching
StrainMedia ConditionSwitching Frequency (%)nNotesa
Wild Type, pMET3-BlankRepressing11.95626
Wild Type, pMET3-WOR1Repressing6.71472
wor4/wor4, pMET3-WOR1Repressing63.13623
wor4/wor4, pMET3-WOR1Repressing64.46436
wor4/wor4, pMET3-WOR1Repressing76.00642
wor4/wor4, pMET3-WOR1Repressing70.01670
  • Opaque-to-white switching on the pMET3-WOR1 wor4 deletion strain was performed using opaque colonies from the white-to-opaque induction assay following several days growth on repressing media to repress WOR1 expression. As explained in the text, the wor4 deletion opaque cells are less stable than normal opaque cells.

  • a 1, Negative control; 2, positive control.