Table 1 Ectopic expression of WOR4 induces white-to-opaque switching
White-to-Opaque Switching
StrainMedia ConditionSwitching Frequency (%)nNotesa
pMET3-BlankRepressing<0.382661
pMET3-BlankInducing<0.402531
pMET3-WOR1Repressing0.401482
pMET3-WOR1Inducing100.001622
pMET3-WOR4Repressing<0.38264
pMET3-WOR4Inducing31.53203
pMET3-RFG1Repressing<0.37269
pMET3-RFG1Inducing<0.51195
Opaque-to-White Switching
StrainMedia ConditionSwitching Frequency (%)nNotesa
pMET3-BlankRepressing0.632051
pMET3-BlankInducing0.882851
pMET3-WOR4Repressing<0.39259
pMET3-WOR4Inducing<1.3972
pMET3-RFG1Repressing1.17309
pMET3-RFG1Inducing0.85117
  • Switching frequency and number of colonies scored for ectopic expression of WOR1, WOR4, and RFG1 under inducing and repressing conditions are indicated.

  • a 1, Negative control; 2, positive control.